Molecular typing and characterization of nasal carriage and community-onset infection methicillin-susceptible Staphylococcus aureus isolates in two Taiwan medical centers.
Molecular typing and characterization of nasal carriage and community-onset infection methicillin-susceptible Staphylococcus aureus isolates in two Taiwan medical centers.
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DOI：
10.1186/1471-2334-12-343
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发表时间：
2012-12-10
影响因子：
3.7
通讯作者：
Lu PL
中科院分区：
文献类型：
--
作者：
Chen FJ;Siu LK;Lin JC;Wang CH;Lu PL
与耐甲氧西林金黄色葡萄球菌(MRSA)相比，甲氧西林敏感金黄色葡萄球菌(MSSA)鼻腔携带和社区感染的特点鲜为人知。台湾此前没有MSSA特征的报道。我们用脉冲场凝胶电泳法(PFGE)、spA分型、多位点序列分型、agr分型、毒力基因检测、生长速度测定和药敏试验对100株鼻腔携带和34株社区感染的MSSA分离株进行了分析。在PFGE分析中，大多数(68%)感染分离株可以以70%的相似性为分界点归入一个主要聚类群。相比之下，只有17%的鼻腔携带分离株属于这一簇。根据SpA类型的重复模式分析，获得了类似的分类。MSSA感染分离物与克隆复合体1(CC1)的毒力克隆密切相关，包括MW2(USA400)和MSSA476。CC1的ST188是检测到的社区首发MSSA感染的优势克隆。在台湾，MSSA和MRSA唯一常见的ST类型是ST59，这是社区相关的MRSA克隆。因此，MRSA很可能是由MSSA克隆通过SCCmec转移而来。与鼻携带株相比，感染株较少携带EGC、TST和HLG基因，对红霉素的敏感率更高(91%比54%)，平均倍增时间更短(38min比55min)。在我们的台湾分离株中，MSSA鼻腔携带株和感染株的克隆谱系不同。大多数社区发病的MSSA感染是由相对较少的克隆谱系引起的。携带EGC、TST和HLG基因的鼻腔携带菌更常见，对红霉素的抗药性更强，生长更慢。
Compared to methicillin-resistant Staphylococcus aureus (MRSA), characteristics of nasal carriage and community-onset infection methicillin-susceptible S. aureus (MSSA) are less well known. No characteristics of MSSA in Taiwan have been reported previously. We analyzed 100 nasal carriage and 34 community-onset infection MSSA isolates by pulsed-field gel electrophoresis (PFGE), spa typing, multi-locus sequence typing, agr typing, virulence gene detection, growth rate measurement, and antimicrobial susceptibility. In PFGE analysis, most (68%) infection isolates could be grouped in one major cluster using a 70% similarity cutoff. In contrast, only 17% of nasal carriage isolates belonged to this cluster. A similar classification was obtained using Based Upon Repeat Pattern analysis of spa types. The MSSA infection isolates cluster was closely related to the virulent clones of clonal complex 1 (CC1), which includes strains MW2 (USA400) and MSSA476. ST188 of CC1 was the predominant clone detected for community-onset MSSA infections. The only common ST type for MSSA and MRSA in Taiwan was ST59, the community-associated MRSA clone. It is likely, therefore, that MRSA originated from MSSA clones through SCCmec transfer. Compared to nasal carriage isolates, infection isolates less frequently possessed egc, tst and hlg genes, were more commonly susceptible to erythromycin (91% vs. 54%), and had shorter mean doubling times (38 min vs. 55 min). The clonal lineages of MSSA nasal carriage and infection isolates differed in our sample of Taiwan isolates. Most community-onset MSSA infections resulted from relatively few clonal lineages. Nasal carriage isolates more frequently possessed the egc, tst and hlg genes, were more resistant to erythromycin, and grew more slowly.
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影响因子：
11.8
作者：
Chen FJ;Lauderdale TL;Huang IW;Lo HJ;Lai JF;Wang HY;Shiau YR;Chen PC;Ito T;Hiramatsu K
通讯作者：
Hiramatsu K
影响因子：
9.4
作者：
Omoe, K;Ishikawa, M;Shinagawa, K
通讯作者：
Shinagawa, K
影响因子：
9.4
作者：
Goering, Richard V.;Shawar, Ribhi M.;Twynholm, Monique E.
通讯作者：
Twynholm, Monique E.
影响因子：
9.4
作者：
Hallin, M.;Deplano, A.;Struelens, M. J.
通讯作者：
Struelens, M. J.
影响因子：
6
作者：
Kuda, T;Shimizu, K;Yano, T
通讯作者：
Yano, T