Complete genome sequences of four isolates of Citrus leaf blotch virus from citrus in China

Complete genome sequences of four isolates of Citrus leaf blotch virus from citrus in China
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中国柑橘四种柑橘叶斑病病毒分离株的全基因组序列

DOI:
10.1016/s2095-3119(17)61860-4
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发表时间:
2018-03
影响因子:
4.8
通讯作者:
Changyong Zhou
Changyong Zhou
中科院分区:
农林科学1区
文献类型:
--
作者:
Ping Li;Min Li;Song Zhang;Jun Wang;Fangyun Yang;Mengji Cao;Changyong Zhou

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柑桔叶斑病毒(Citrus leaf blotch virus,CLBV)是Betaflexiviridae柑桔病毒属Citrivirus属的成员,在我国已报道可侵染猕猴桃、柑桔和甜樱桃。从我国6个地区的289份柑橘样品中,检出15份感染CLBV。获得了4个CLBV分离物的全基因组序列,分别来自四川热口(CLBV-LH)、浙江玉拉禾色(CLBV-YL)、湖南冰糖城(CLBV-BT)和重庆奉节72-1(CLBV-FJ)。除poly(A)尾外,所有分离株均编码3个开放阅读框(ORF),单片段长度为8747个核苷酸。基因组多重比对结果表明,4个分离物的同源性为98.9 ~ 99.8%,与GenBank中柑橘属参考文献的同源性为96.8 ~ 98.1%。基于基因组序列构建的系统发育树表明,4个分离物聚在一起,表明中国柑橘CLBV分离物之间没有明显的变异。这是首次报道我国柑桔CLBV分离物的全序列。
Citrus leaf blotch virus(CLBV) is a member of the genusCitrivirus,in the familyBetaflexiviridae.It has been reported CLBV could infect kiwi, citrus and sweet cherry in China. Of 289 citrus samples from six regions of China, 15 were detected to be infected with CLBV in this study. The complete genome of four isolates of CLBV was obtained from Reikou in Sichuan (CLBV-LH), Yura Wase in Zhejiang (CLBV-YL), Bingtangcheng in Hunan (CLBV-BT), Fengjie 72-1 in Chongqing (CLBV-FJ), respectively. While they all represented 8 747 nucleotides in monopartite size, excluding the poly(A) tail, each of the isolates coded three open reading frames (ORFs). Identity of the four isolates ranged from 98.9 to 99.8% to each other and from 96.8 to 98.1% to the citrus references in GenBank by multiple alignment of genomes. A phylogenetic tree based on the genome sequences of available CLBV isolates indicated that the four isolates were clustered together, suggesting that CLBV isolates from citrus in China did not have obvious variation. This is the first report of the complete nucleotide sequences of CLBV isolates infecting citrus in China.
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