NXSensor web tool for evaluating DNA for nucleosome exclusion sequences and accessibility to binding factors.
NXSensor web tool for evaluating DNA for nucleosome exclusion sequences and accessibility to binding factors.
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DOI：
10.1093/nar/gkl158
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发表时间：
2006-07-01
影响因子：
14.9
通讯作者：
Khuri S
中科院分区：
文献类型：
--
作者：
Luykx P;Bajić IV;Khuri S
核小体是真核生物染色质的基本结构单位，在基因表达调控中起着重要作用。我们已经开发了一个网络工具的基础上已知的DNA序列从经验和理论研究影响DNA弯曲和灵活性，并排除核小体。NXSensor（可在）发现核小体排除序列，评估其长度和间距，并计算“可及性得分”，给出可能无核小体的碱基对的比例。将NXSensor应用于管家（HK）基因和组织特异性（TS）基因的启动子区域，发现两类基因之间存在显著差异，平均而言，前者明显更开放，特别是在转录起始位点（TSS）附近。NXSensor应该是一个有用的工具，用于评估基因调控和染色质功能其他方面相关区域中核小体形成的可能性。
Nucleosomes, a basic structural unit of eukaryotic chromatin, play a significant role in regulating gene expression. We have developed a web tool based on DNA sequences known from empirical and theoretical studies to influence DNA bending and flexibility, and to exclude nucleosomes. NXSensor (available at ) finds nucleosome exclusion sequences, evaluates their length and spacing, and computes an ‘accessibility score’ giving the proportion of base pairs likely to be nucleosome-free. Application of NXSensor to the promoter regions of housekeeping (HK) genes and those of tissue-specific (TS) genes revealed a significant difference between the two classes of gene, the former being significantly more open, on average, particularly near transcription start sites (TSSs). NXSensor should be a useful tool in assessing the likelihood of nucleosome formation in regions involved in gene regulation and other aspects of chromatin function.
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影响因子：
2.9
作者：
Morse, RH
通讯作者：
Morse, RH
影响因子：
3.8
作者：
Powell, NG;Ferreiro, J;Waters, R
通讯作者：
Waters, R
影响因子：
3
作者：
Ganapathi M;Srivastava P;Das Sutar SK;Kumar K;Dasgupta D;Pal Singh G;Brahmachari V;Brahmachari SK
通讯作者：
Brahmachari SK
影响因子：
5.8
作者：
Levitsky, VG;Podkolodnaya, OA;Podkolodny, NL
通讯作者：
Podkolodny, NL
影响因子：
14.9
作者：
Levitsky VG;Katokhin AV;Podkolodnaya OA;Furman DP;Kolchanov NA
通讯作者：
Kolchanov NA