Straw- and slurry-associated prokaryotic communities differ during co-fermentation of straw and swine manure
Straw- and slurry-associated prokaryotic communities differ during co-fermentation of straw and swine manure
复制标题
秸秆和猪粪共发酵过程中秸秆和粪浆相关的原核生物群落存在差异
DOI：
10.1007/s00253-014-5629-3
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发表时间：
2014-03
影响因子：
5
通讯作者：
Li, Xiangzhen
中科院分区：
文献类型：
--
作者：
Wang, Yuanpeng;Liu, Xiaofeng;Zheng, Tao;Li, Xiangzhen
秸秆和粪便的厌氧共发酵被广泛用于废物处理和沼气生产。然而，秸秆和泥浆相关的原核生物群落之间的差异，它们在整个共发酵过程中的动态变化，以及它们与生物反应器性能的相关性还没有完全了解。为了解决这些问题，我们研究了原核生物的社区组成和原核生物附着在秸秆和浆料中的小麦秸秆和猪粪共发酵过程中，利用焦磷酸测序技术的动态。结果表明，秸秆和泥浆相关的原核生物在结构和功能上是不同的。秸秆相关的原核生物群落的门螺旋体和Fibrobacteres，而Synergistetes和Euryarchaeota更丰富的泥浆中。与秸秆相关的候选类别TG3、纤维杆菌属、拟杆菌属、醋弧菌属、梭菌属III、乳头状杆菌属、密螺旋体属、沉降杆菌属和Lutispora属可能专门从事底物水解。丙酸是最丰富的挥发性脂肪酸的浆料中，它可能是通过互养氧化由属Pelotomaculum，Methanoculleus，和Methanosaeta降解。蛋白质发酵菌Aminobacterium和Cloacibacillus在料浆中含量丰富，表明蛋白质是共发酵的重要底物。这项研究提供了一个更好的了解厌氧共发酵过程，是由空间差异化的微生物群驱动。
Anaerobic co-fermentation of straw and manure is widely used for waste treatment and biogas production. However, the differences between the straw- and slurry-associated prokaryotic communities, their dynamic changes throughout the co-fermentation process, and their correlations with bioreactor performance are not fully understood. To address these questions, we investigated the prokaryotic community compositions and the dynamics of prokaryotes attached to the straw and in the slurry during co-fermentation of wheat straw and swine manure using pyrosequencing technique. The results showed that straw- and slurry-associated prokaryotes were different in their structure and function. Straw-associated prokaryotic communities were overrepresented by the phyla Spirochaetes and Fibrobacteres, while Synergistetes and Euryarchaeota were more abundant in the slurry. The straw-associated candidate class TG3, genera Fibrobacter, Bacteroides, Acetivibrio, Clostridium III, Papillibacter, Treponema, Sedimentibacter, and Lutispora may specialize in substrate hydrolysis. Propionate was the most abundant volatile fatty acid in the slurry, and it was probably degraded through syntrophic oxidation by the genera Pelotomaculum, Methanoculleus, and Methanosaeta. The protein-fermenting bacteria Aminobacterium and Cloacibacillus were much abundant in the slurry, indicating that proteins are important substrates in the co-fermentation. This study provided a better understanding of the anaerobic co-fermentation process that is driven by spatially differentiated microbiota.
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DOI：
10.1099/ijs.0.044719-0
发表时间：
2013-06
影响因子：
2.8
作者：
Looft T;Levine UY;Stanton TB
通讯作者：
Stanton TB
影响因子：
6.3
作者：
Li A;Chu Y;Wang X;Ren L;Yu J;Liu X;Yan J;Zhang L;Wu S;Li S
通讯作者：
Li S
影响因子：
7.7
作者：
McInerney, Michael J.;Sieber, Jessica R.;Gunsalus, Robert P.
通讯作者：
Gunsalus, Robert P.
影响因子：
11
作者：
Imachi, Hiroyuki;Aoi, Ken;Takai, Ken
通讯作者：
Takai, Ken
影响因子：
11.4
作者：
Nelson, Michael C.;Morrison, Mark;Yu, Zhongtang
通讯作者：
Yu, Zhongtang