Inactivation of median preoptic nucleus causes c-Fos expression in hypocretin- and serotonin-containing neurons in anesthetized rat.
Inactivation of median preoptic nucleus causes c-Fos expression in hypocretin- and serotonin-containing neurons in anesthetized rat.
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DOI：
10.1016/j.brainres.2008.07.115
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发表时间：
2008-10-09
期刊：
影响因子：
2.9
通讯作者：
Alam MN
中科院分区：
文献类型：
--
作者：
Kumar S;Szymusiak R;Bashir T;Suntsova N;Rai S;McGinty D;Alam MN
下丘脑的正中视前核（MnPN）含有睡眠活性神经元，包括睡眠活性GABA能神经元，并参与非REM/REM睡眠的调节。下丘脑穹窿周围外侧区（PF-LHA）的下丘脑泌素能（HCRT）神经元和中缝背核（DRN）的多巴胺能（5-HT）神经元在清醒时大多活跃，并参与觉醒的调节。MnPN GABA能神经元投射到PF-LHA和DRN。据推测，MnPN通过抑制多种唤醒系统来促进睡眠，包括PF-LHA内的HCRT和其他唤醒活性神经元以及DRN中的5-HT神经元。我们通过在麻醉大鼠的MnPN中局部微量注射0.2 μl的1 mM或10 mM GABAA受体激动剂蝇蕈醇溶液来检测MnPN神经元失活对PF-LHA神经元（包括DRN中的HCRT神经元和5-HT神经元）中Fos表达（Fos-IR）的影响。与人工脑脊液对照组相比，MnPN内微量注射蝇蕈醇后，PF-LHA内HCRT和non-HCRT神经元以及DRN内5-HT神经元中Fos阳性神经元的比例显著升高，而PF-LHA内黑色素浓集激素（MCH）+/Fos+神经元的比例无明显变化。这些结果支持了一个假设，即MnPN神经元的激活有助于抑制促醒系统，包括HCRT和其他未识别的神经元在PF-LHA和5-HT神经元在DRN。这些结果还表明，MCH神经元可能不受MnPN抑制控制。这些发现与MnPN在睡眠调节中的假设作用一致。
The median preoptic nucleus (MnPN) of the hypothalamus contains sleep-active neurons including sleep-active GABAergic neurons and is involved in the regulation of nonREM/REM sleep. The hypocretinergic (HCRT) neurons of the perifornical-lateral hypothalamic area (PF-LHA) and serotonergic (5-HT) neurons of the dorsal raphe nucleus (DRN) are mostly active during waking and have been implicated in the regulation of arousal. MnPN GABAergic neurons project to the PF-LHA and DRN. It is hypothesized that MnPN promotes sleep by inhibiting multiple arousal systems including HCRT and other wake-active neurons within the PF-LHA and 5-HT neurons in the DRN. We examined the effects of inactivation of MnPN neurons by locally microinjecting 0.2 µl of 1mM or 10mM solutions of a GABAA receptor agonist, muscimol, into the MnPN on Fos expression (Fos-IR) in the PF-LHA neurons including HCRT neurons and 5-HT neurons in the DRN in anesthetized rats. Compared to artificial cerebrospinal fluid control, microinjection of muscimol into the MnPN resulted in significantly higher percentages of HCRT and non-HCRT neurons in the PF-LHA and 5-HT neurons in the DRN that exhibited Fos-IR. The percentage of melanin-concentrating hormone (MCH)+/Fos+ neurons in the PF-LHA did not change after muscimol treatments. These results support a hypothesis that the activation of MnPN neurons contributes to the suppression of wake-promoting systems including HCRT and other unidentified neurons in the PF-LHA and 5-HT neurons in the DRN. These results also suggest that MCH neurons may not be under MnPN inhibitory control. These findings are consistent with a hypothesized role of MnPN in sleep regulation.
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影响因子：
16.2
作者：
Li, Y;Gao, XB;van den Pol, AN
通讯作者：
van den Pol, AN
DOI：
10.1152/ajpregu.2000.279.6.r2079
发表时间：
2000-12-01
影响因子：
2.8
作者：
Gong, H;Szymusiak, R;McGinty, D
通讯作者：
McGinty, D
影响因子：
3.3
作者：
España, RA;Baldo, BA;Berridge, CW
通讯作者：
Berridge, CW
影响因子：
2.9
作者：
Guzmán-Marín, R;Alam, MN;McGinty, D
通讯作者：
McGinty, D
影响因子：
5.3
作者：
Lee, MG;Hassani, OK;Jones, BE
通讯作者：
Jones, BE