Nuclear movement in fungi.
Nuclear movement in fungi.
复制标题
真菌的核运动。
DOI：
10.1016/j.semcdb.2017.10.024
复制
发表时间：
2018-10
影响因子：
7.3
通讯作者：
Xiang X
中科院分区：
文献类型：
--
作者：
Xiang X
细胞内的核运动发生在各种真核生物中，包括酵母菌和丝状真菌。真菌分子遗传学研究证实，负端定向的微管马达细胞质动力蛋白是核运动或核内有丝分裂纺锤体定位的关键蛋白。对发芽酵母的研究首次表明，动力蛋白通过其锚定蛋白Num1锚定在皮层上，对星体微管施加拉力，使后期纺锤体在核分裂之前穿过母子轴。在后期之前，肌球蛋白V通过Kar9-Bim1/EB1与星形微管的正端相互作用，并沿着肌动蛋白缆线拉动正端，使核/纺锤体靠近芽颈。此外，由皮质相关的微管聚合或解聚产生的推力或拉力推动了酵母中的核运动，可能也驱动了丝状真菌中的核运动。在丝状真菌中，菌丝段内的多个细胞核进行动力蛋白依赖的来回移动，它们的位置也受到细胞质流向菌丝顶端的影响。此外，核运动发生在真菌发育和植物组织的真菌感染的不同阶段。这篇综述讨论了我们目前对真菌生物体中核运动机制、核定位的重要性以及确保核/纺锤体正确定位的调控策略的理解。
Nuclear movement within a cell occurs in a variety of eukaryotic organisms including yeasts and filamentous fungi. Fungal molecular genetic studies identified the minus-end-directed microtubule motor cytoplasmic dynein as a critical protein for nuclear movement or orientation of the mitotic spindle contained in the nucleus. Studies in the budding yeast first indicated that dynein anchored at the cortex via its anchoring protein Num1 exerts pulling force on an astral microtubule to orient the anaphase spindle across the mother-daughter axis before nuclear division. Prior to anaphase, myosin V interacts with the plus end of an astral microtubule via Kar9-Bim1/EB1 and pulls the plus end along the actin cables to move the nucleus/spindle close to the bud neck. In addition, pushing or pulling forces generated from cortex-linked polymerization or depolymerization of microtubules drive nuclear movements in yeasts and possibly also in filamentous fungi. In filamentous fungi, multiple nuclei within a hyphal segment undergo dynein-dependent back-and-forth movements and their positioning is also influenced by cytoplasmic streaming toward the hyphal tip. In addition, nuclear movement occurs at various stages of fungal development and fungal infection of plant tissues. This review discusses our current understanding on the mechanisms of nuclear movement in fungal organisms, the importance of nuclear positioning and the regulatory strategies that ensure the proper positioning of nucleus/spindle.
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DOI：
10.1083/jcb.138.3.629
发表时间：
1997-08-11
期刊：
The Journal of cell biology
影响因子：
--
作者：
Carminati JL;Stearns T
通讯作者：
Stearns T
影响因子：
2.9
作者：
Alonso, Annabel;Greenlee, Matt;Matts, Jessica;Kline, Jake;Davis, Kayla J.;Miller, Rita K.
通讯作者：
Miller, Rita K.
影响因子：
9.2
作者：
Beach, DL;Thibodeaux, J;Bloom, K
通讯作者：
Bloom, K
影响因子：
64.5
作者：
Ananthanarayanan, Vaishnavi;Schattat, Martin;Tolic-Norrelykke, Iva M.
通讯作者：
Tolic-Norrelykke, Iva M.
DOI：
10.1083/jcb.143.5.1239
发表时间：
1998-11-30
期刊：
The Journal of cell biology
影响因子：
--
作者：
Beckwith SM;Roghi CH;Liu B;Ronald Morris N
通讯作者：
Ronald Morris N