Variations of glucose metabolism by fat cells from three adipose depots of the rat.
Variations of glucose metabolism by fat cells from three adipose depots of the rat.
复制标题
大鼠三个脂肪库脂肪细胞葡萄糖代谢的变化。
DOI：
10.1016/0026-0495(82)90176-7
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Pi-Sunyer,FX
中科院分区：
文献类型：
--
作者：
Fried,SK;Lavau,M;Pi-Sunyer,FX
从150g雄性Wistar大鼠的附睾(E)和背皮下(S)库分离的脂肪细胞大小相似，但葡萄糖代谢明显不同。腹膜后（RP）脂肪细胞稍大，但代谢与附睾脂肪细胞相似。[1-14C]葡萄糖在脂肪酸中的基础掺入量在S组低于E组和RP组，三者的co2产量和甘油-甘油合成相似；与RP和E脂肪细胞相比，S脂肪细胞在葡萄糖并入脂肪酸、二氧化碳和乳酸生成方面对胰岛素的亚最大和最大刺激浓度均表现出迟钝的反应，但对甘油-甘油没有反应。胰岛素刺激下S脂肪细胞脂肪酸合成的最大值分别为E和RP脂肪细胞的19%和29%。与E和RP相比，S细胞中基础和最大程度胰岛素刺激的葡萄糖转运（2-脱氧[14C]葡萄糖摄取）被抑制了30%-40%。因此，S的基础葡萄糖利用率下降可能主要归因于葡萄糖运输能力下降。然而，胰岛素刺激的S脂肪细胞葡萄糖代谢明显降低，可能是由于转运能力和细胞内代谢能力的改变。皮下脂肪细胞对次最大剂量的胰岛素也不太敏感，这反映在2-脱氧葡萄糖摄取和脂肪酸合成的剂量-反应曲线的右移上。胰岛素刺激的葡萄糖氧化和脂肪酸合成的减少与主要脂肪生成酶的减少是平行的。尽管不同脂肪组织之间葡萄糖代谢能力差异的原因尚不清楚，但它们对评估整个脂肪器官的代谢功能很重要。
Fat cells isolated from the epididymal (E) and dorsal subcutaneous (S) depots from 150g male Wistar rats were similar in size, but differed markedly in glucose metabolism. Retroperitoneal (RP) fat cells were slightly larger but were metabolically similar to epididymal fat cells. Basal incorporation of [1-14C] glucose into fatty acids was lower in S than E and RP, CO2production and glyceride-glycerol synthesis were similar in all three; and lactate production was increased in RP and S compared to E. S adipocytes exhibited a blunted respond to both submaximally and maximally-stimulating concentrations of insulin compared to RP and E adipocytes in glucose incorporation into fatty acids, CO2and lactate production, but not glyceride-glycerol. Maximally insulin-stimulated fatty acid synthesis by S fat cells was 19% and 29% of the values in E and RP fat cells respectively. Basal and maximally insulin-stimulated glucose transport (2-deoxy[14C] glucose uptake) was depressed by 30%–40% in S cells compared to E and RP. Thus, the decreased basal glucose utilization of S could be attributed primarily to a decreased glucose transport capacity. The markedly lower insulin-stimulated glucose metabolism in S fat cells, however, may be explained by alterations of the capacities for both transport and intracellular metabolism. Subcutaneous fat cells were also somewhat less sensitive to submaximal doses of insulin and this was reflected in rightward shift in the dose-response curves for 2-deoxyglucose uptake and fatty acid synthesis. The decreases in insulin stimulated glucose oxidation and fatty acid synthesis were paralleled by decreases in the major lipogenic enzymes. Although the reason for these variations in the capacity for glucose metabolism among depots is unknown, they are important in assessing the metabolic function of the whole adipose organ.
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影响因子：
4.1
作者：
JAMDAR, SC
通讯作者：
JAMDAR, SC
雌激素对大鼠局部脂肪组织细胞结构的影响。
DOI：
10.1111/j.1748-1716.1976.tb10178.x
发表时间：
1976
期刊：
Acta physiologica Scandinavica
影响因子：
--
作者：
Marcin Krotkiewski
通讯作者：
Marcin Krotkiewski
衰老对大鼠脂肪组织脂质组成及代谢的影响
DOI：
--
发表时间：
1961
期刊：
影响因子：
--
作者：
W. Benjamin;A. Gellhorn;M. Wagner;H. Kundel
通讯作者：
H. Kundel
新西兰肥胖小鼠脂肪细胞的位点差异——一项移植研究。
DOI：
10.1016/0026-0495(80)90124-9
发表时间：
1980
期刊：
Metabolism: clinical and experimental
影响因子：
--
作者：
C. J. Meade;M. Ashwell
通讯作者：
M. Ashwell
大鼠脂肪组织中脂蛋白脂肪酶的分布：对乳糜微粒摄取的影响。
DOI：
10.1152/ajpendo.1977.232.3.e316
发表时间：
1977
期刊：
The American journal of physiology
影响因子：
--
作者：
A. D. Hartman
通讯作者：
A. D. Hartman