Antioxidant and antiproliferative activities of anthocyanin/ellagitannin-enriched extracts from Syzygium cumini L. (Jamun, the Indian Blackberry).
Antioxidant and antiproliferative activities of anthocyanin/ellagitannin-enriched extracts from Syzygium cumini L. (Jamun, the Indian Blackberry).
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DOI：
10.1080/01635581.2012.657766
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发表时间：
2012-04
期刊：
影响因子：
--
通讯作者：
Gupta RC
中科院分区：
文献类型：
--
作者：
Aqil F;Gupta A;Munagala R;Jeyabalan J;Kausar H;Sharma RJ;Singh IP;Gupta RC
有色水果，特别是浆果，具有高度的化学保护作用，因为它们具有抗氧化，抗增殖和抗炎活性。我们报道了蒲桃的癌症化学保护潜力，俗称“jamun”或印度黑莓。采用酸性乙醇提取花色苷等多酚类化合物，用Amberlite XAD 7/HP 20（1：1）富集。发现果肉粉末含有0.54%的花青素、0.17%的鞣花酸/鞣花单宁和1.15%的多酚。Jamun种子含有没有检测到的花青素，但有较高的量的鞣花酸/鞣花单宁（0.5%）和总多酚（2.7%）比纸浆粉。酸水解后，果肉提取物经HPLC分析得到5种花青素：锦葵色素（44.4%）、矮牵牛色素（24.2%）、飞燕草色素（20.3%）、矢车菊色素（6.6%）和芍药色素（2.2%）。果浆提取物（1，445 ±64 μmol trolox当量（TE）/g）和种子提取物（3，379 ±151 μM TE/g）均表现出较高的氧自由基吸收能力（ORAC）。它们的抗氧化能力还表现在2，2 ′-连氮基-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS）-和2，2-二苯基-1-苦基肼（DPPH）-清除能力以及亚铁离子螯合能力。我们还分析了jamun提取物对人肺癌A549细胞的抗增殖活性。水解果肉和种子提取物显示出显着的抗增殖活性。然而，未水解的提取物显示出低得多的活性。这些数据表明，除了五个花青素，jamun含有相当数量的鞣花酸/鞣花单宁，具有高的抗氧化和抗增殖活性。
Colored fruits, particularly berries, are highly chemoprotective because of their antioxidant, anti-proliferative and anti-inflammatory activities. We report cancer chemoprotective potential of Syzygium cumini L., commonly known as ‘jamun’ or Indian blackberry. Anthocyanins and other polyphenolics were extracted with acidic ethanol, and enriched by amberlite XAD7/HP20 (1:1). The pulp powder was found to contain 0.54% anthocyanins, 0.17% ellagic acid/ellagitannins and 1.15% polyphenolics. Jamun seed contained no detectable anthocyanins, but had higher amounts of ellagic acid/ellagitannins (0.5%) and total polyphenolics (2.7%) than the pulp powder. Upon acid hydrolysis, the pulp extract yielded five anthocyanidins by HPLC: malvidin (44.4%), petunidin (24.2%), delphinidin (20.3%), cyanidin (6.6%), and peonidin (2.2%). Extracts of both jamun pulp (1,445±64 μmol of trolox equivalent (TE)/g) and seeds (3,379±151 μM of TE/g) showed high oxygen radical absorbance capacity (ORAC). Their high antioxidant potential was also reflected by 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)- and 2,2-diphenyl-1-picrylhydrazyl (DPPH)-scavenging, and ferrous ion-chelating activities. We also analyzed anti-proliferative activity of jamun extracts against human lung cancer A549 cells. The hydrolyzed pulp and seed extracts showed significant antiproliferative activity. However, unhydrolyzed extracts showed much less activity. These data showed that in addition to five anthocyanidins, jamun contains appreciable amounts of ellagic acid/ellagitannins, with high antioxidant and antiproliferative activities.
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影响因子：
6.1
作者：
Liu, F;Ng, TB
通讯作者：
Ng, TB
影响因子：
4.7
作者：
Munagala, Radha;Kausar, Hina;Gupta, Ramesh C.
通讯作者：
Gupta, Ramesh C.
影响因子：
4.1
作者：
Kähkönen, MP;Heinämäki, J;Heinonen, M
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Heinonen, M
影响因子：
5.4
作者：
Arun, Renganathan;Prakash, M. Velayutham Dass;Premkumar, Kumpati
通讯作者：
Premkumar, Kumpati
影响因子：
3.6
作者：
Andres-Lacueva, C;Shukitt-Hale, B;Joseph, JA
通讯作者：
Joseph, JA