Reduced pain and inflammation in juvenile and adult rats fed a ketogenic diet.
Reduced pain and inflammation in juvenile and adult rats fed a ketogenic diet.
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DOI：
10.1371/journal.pone.0008349
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发表时间：
2009-12-23
期刊：
影响因子：
3.7
通讯作者：
Masino SA
中科院分区：
文献类型：
--
作者：
Ruskin DN;Kawamura M;Masino SA
生酮饮食是一种高脂肪、低碳水化合物的饮食方案，强制基于酮而不是基于葡萄糖的细胞新陈代谢。在临床上，生酮饮食已被证明在治疗儿童癫痫和II型糖尿病方面有效，最近的基础研究提供了证据，表明生酮策略在减少脑损伤方面有希望。生酮饮食治疗成功的细胞机制，如活性氧的减少和中枢腺苷的增加，提示饮食诱导的酮类代谢可以减轻疼痛和炎症。为了直接测试以酮为基础的代谢对疼痛和炎症的影响，我们给幼年和成年大鼠喂以对照饲料(标准啮齿动物饲料)或生酮饲料(79%脂肪)，随机喂养3-4周。然后，我们量化了后爪热伤害感作为疼痛指标，并完成了弗洛因德佐剂诱导的局部后爪肿胀和血浆外渗(血管中的液体运动)作为炎症指标。与年龄无关，维持生酮饮食显著降低了外周炎症反应，通过测量爪子肿胀和血浆外渗来衡量。生酮饮食还诱导了显著的热痛觉减退，不依赖于年龄，表现为在热板伤害性试验中后爪撤退潜伏期延长。在青少年中，抗炎和止痛的饮食效果通常更强。生酮饮食在两个年龄组中升高血浆酮的作用相似，但仅在青少年中导致身体发育减慢。这些数据表明，应用生酮饮食或利用与酮类代谢相关的细胞机制为控制疼痛和外周炎症提供了新的治疗机会，这样的代谢策略可能会为儿童和成人带来显著的好处。
The ketogenic diet is a high-fat, low-carbohydrate regimen that forces ketone-based rather than glucose-based cellular metabolism. Clinically, maintenance on a ketogenic diet has been proven effective in treating pediatric epilepsy and type II diabetes, and recent basic research provides evidence that ketogenic strategies offer promise in reducing brain injury. Cellular mechanisms hypothesized to be mobilized by ketone metabolism and underlying the success of ketogenic diet therapy, such as reduced reactive oxygen species and increased central adenosine, suggest that the ketolytic metabolism induced by the diet could reduce pain and inflammation. To test the effects of a ketone-based metabolism on pain and inflammation directly, we fed juvenile and adult rats a control diet (standard rodent chow) or ketogenic diet (79% fat) ad libitum for 3–4 weeks. We then quantified hindpaw thermal nociception as a pain measure and complete Freund's adjuvant-induced local hindpaw swelling and plasma extravasation (fluid movement from the vasculature) as inflammation measures. Independent of age, maintenance on a ketogenic diet reduced the peripheral inflammatory response significantly as measured by paw swelling and plasma extravasation. The ketogenic diet also induced significant thermal hypoalgesia independent of age, shown by increased hindpaw withdrawal latency in the hotplate nociception test. Anti-inflammatory and hypoalgesic diet effects were generally more robust in juveniles. The ketogenic diet elevated plasma ketones similarly in both age groups, but caused slowed body growth only in juveniles. These data suggest that applying a ketogenic diet or exploiting cellular mechanisms associated with ketone-based metabolism offers new therapeutic opportunities for controlling pain and peripheral inflammation, and that such a metabolic strategy may offer significant benefits for children and adults.
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影响因子：
2
作者：
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通讯作者：
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DOI：
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发表时间：
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影响因子：
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