Chronic stress effects on dendritic morphology in medial prefrontal cortex: sex differences and estrogen dependence.
Chronic stress effects on dendritic morphology in medial prefrontal cortex: sex differences and estrogen dependence.
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DOI：
10.1016/j.neuroscience.2009.04.057
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发表时间：
2009-08-04
期刊：
影响因子：
3.3
通讯作者：
Wellman, C. L.
中科院分区：
文献类型：
--
作者：
Garrett, J. E.;Wellman, C. L.
越来越多的研究表明，在对压力的行为、神经化学和形态反应方面，性别存在差异。慢性应激改变雄性大鼠内侧前额叶皮质树突形态。然而，潜在的性别差异，在内侧前额叶皮层的压力引起的形态学变化还没有被检查。因此，在实验1中，我们评估了慢性应激后雄性和雌性大鼠内侧前额叶皮层的树突形态。雄性和雌性大鼠每天进行3小时的束缚，持续一周，或者除了称重外不进行处理。在束缚的最后一天，对所有大鼠实施安乐死，并使用Golgi-Cox程序对大脑进行染色。在内侧前额叶皮层的第二-第三层的锥体神经元绘制在三维空间，和形态学的顶端和基底动脉的乔木进行了量化。在男性中，应力减少顶端树突分支的数量和长度，而在女性中，应力增加顶端树突的长度。在实验2中，我们通过评估束缚应激对接受卵巢切除加或不加17-β-雌二醇替代或假卵巢切除的雌性大鼠的影响来评估雌二醇是否介导雌性大鼠中这种应激诱导的树突肥大。如实验1中所述处理脑并重建神经元。假手术和卵巢切除的大鼠雌二醇植入物表现出应力诱导的顶端树突状材料的增加，而卵巢切除术没有雌二醇替代防止应力诱导的增加。因此，在女性的顶端树突材料的应力诱导的增加是雌二醇依赖性的。
A growing body of work has documented sex differences in many behavioral, neurochemical, and morphological responses to stress. Chronic stress alters morphology of dendrites in medial prefrontal cortex in male rats. However, potential sex differences in stress-induced morphological changes in medial prefrontal cortex have not been examined. Thus, in Experiment 1 we assessed dendritic morphology in medial prefrontal cortex in male and female rats after chronic stress. Male and female rats underwent either three hours of restraint daily for one week or were left unhandled except for weighing. On the final day of restraint, all rats were euthanized and brains were stained using a Golgi-Cox procedure. Pyramidal neurons in layer II-III of medial prefrontal cortex were drawn in three dimensions, and morphology of apical and basilar arbors was quantified. In males, stress decreased apical dendritic branch number and length, whereas in females, stress increased apical dendritic length. In Experiment 2, we assessed whether estradiol mediates this stress-induced dendritic hypertrophy in females by assessing the effects of restraint stress on female rats that had received either ovariectomy with or without 17-β-estradiol replacement or sham ovariectomy. Brains were processed and neurons reconstructed as described in Experiment 1. Both sham-operated and ovariectomized rats with estradiol implants showed stress-induced increases in apical dendritic material, whereas ovariectomy without estradiol replacement prevented the stress-induced increase. Thus, the stress-induced increase in apical dendritic material in females is estradiol-dependent.
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