Qianliening capsule treats benign prostatic hyperplasia (BPH) by down-regulating the expression of PCNA, CyclinD1 and CDK4
Qianliening capsule treats benign prostatic hyperplasia (BPH) by down-regulating the expression of PCNA, CyclinD1 and CDK4
复制标题
前列宁胶囊通过下调PCNA、CyclinD1、CDK4表达治疗良性前列腺增生(BPH)
DOI：
10.4314/ajb.v11i30
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发表时间：
2012-04
影响因子：
--
通讯作者：
Jun Peng
中科院分区：
文献类型：
--
作者：
Xiaoyong Zhong;Jiumao Lin;Jianhng Zhou;Wei Xu;Zhenfeng Hong;Jun Peng
良性前列腺增生（BPH）是前列腺组织中上皮细胞和间质细胞异常增殖的特征性疾病，其发生与PCNA、CyclinD 1和CDK 4表达增加密切相关。因此，通过抑制上述基因的表达来抑制细胞增殖是开发新型抗BPH疗法的有前景的策略。本研究旨在探讨前列宁胶囊（QC）对BPH大鼠前列腺组织中PCNA、CyclinD 1和CDK 4表达的影响。雄性SD大鼠去势后皮下注射丙酸睾酮建立BPH模型。同时，BPH大鼠经口给予QC或作为阳性对照药物的非那肽。QC或非那肽能显著降低BPH大鼠的PI（前列腺指数，PI =前列腺湿重/体重× 100%）（P <0.05）。此外，QC或非那肽治疗可显著抑制模型诱导的BPH大鼠前列腺组织PCNA、CyclinD 1和CDK 4表达的上调（P <0.05）。本研究首次证实了清前列腺素能明显降低BPH大鼠前列腺组织PI、PCNA、CyclinD 1和CDK 4的表达，这可能是清前列腺素抗BPH作用的部分原因。关键词：前列宁胶囊，良性前列腺增生，细胞增殖，PCNA，CyclinD 1，CDK 4
Benign prostatic hyperplasia (BPH) is characterized by abnormal proliferation of epithelial and stromal cells in prostatic tissue, which is closely correlated with increased expression of PCNA, CyclinD1 and CDK4. Therefore, inhibition of cell proliferation by suppressing the expression of the above genes is a promising strategy in the development of novel anti-BPH therapies. The aim of this study was to investigate the effect of Qianliening capsule (QC), a traditional Chinese formulation that has been shown to be clinically effective in the treatment of BPH, on the expression of PCNA, CyclinD1 and CDK4 in prostatic tissues of BPH rats. Male Sprage-Dawley (SD) rats were castrated and subcutaneously injected with testosterone propionate to generate BPH model. Meanwhile, BPH rats were orally treated with QC, or with finasteride that was used as a positive control drug. Treatment with QC or finasteride significantly reduced the PI (Prostate Index, PI = prostate wet weight / body weight × 100%) in BPH rats ( P <0.05). In addition, QC or finasteride treatment significantly inhibited model construction-induced upregulation of expression of PCNA, CyclinD1 and CDK4 in prostatic tissues of BPH rats ( P <0.05). Our findings for the first time demonstrated that QC can obviously reduce the PI, the expression of PCNA, CyclinD1 and CDK4 in the prostatic tissues of BPH rats, which may in part explain its anti-BPH activity. Keywords: Qianliening capsule, benign prostatic hyperplasia, cell proliferation, PCNA, CyclinD1, CDK4
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DOI：
--
发表时间：
2010
期刊：
Journal of Fujian University of Traditional Chinese Medicine
影响因子：
--
作者：
Zhao Jin-yan
通讯作者：
Zhao Jin-yan
影响因子：
8.9
作者：
Lieber, MM
通讯作者：
Lieber, MM
DOI：
--
发表时间：
2010
期刊：
Chinese Archives of Traditional Chinese Medicine
影响因子：
--
作者：
Hong Zhen-feng
通讯作者：
Hong Zhen-feng
影响因子：
--
作者：
Min-Dao Zhang;Yong-Ning Zhao;Li-wen An
通讯作者：
Min-Dao Zhang;Yong-Ning Zhao;Li-wen An
DOI：
--
发表时间：
2008
期刊：
Journal of Fujian University of Traditional Chinese Medicine
影响因子：
--
作者：
Lin Jiu-mao
通讯作者：
Lin Jiu-mao