Molecular detection and genomic characterization of porcine circovirus 3 in pigs from Northeast China.
Molecular detection and genomic characterization of porcine circovirus 3 in pigs from Northeast China.
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DOI：
10.1186/s12917-018-1634-6
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发表时间：
2018-10-26
影响因子：
2.6
通讯作者：
Jin NY
中科院分区：
文献类型：
--
作者：
Ha Z;Xie CZ;Li JF;Wen SB;Zhang KL;Nan FL;Zhang H;Guo YC;Wang W;Lu HJ;Jin NY
猪圆环病毒3型(PCV3)于2016年在美国首次发现，是一种新出现的猪圆环病毒，表现出广泛的临床症状，这可能与在猪身上观察到的致病性有关。本研究的目的是鉴定和分析我国东北地区流行的猪圆环病毒中国株的全基因组序列。在此，对2018年从东北中国病猪身上分离的105份肺标本进行了PCV3型病毒的检测。用聚合酶链式反应检测PCV3总阳性率为33.3%(35/105)，黑龙江、吉林、辽宁分别为17.8%(8/45)、66.7%(10/15)、37.8%(17/45)。此外，我们的研究结果显示，PCV3阳性样本与PCV2、PPV6和PPV7的混合感染率很高。为了研究中国东北地区新城疫病毒中国的进化，我们测定了13株新城疫病毒的全基因组序列。系统发育分析结果表明，PCV3可分为PCV3a和PCV3b两个分支。有趣的是，在1999年PCV3-CN2018LN-4(MH277118)基因组中发现了1072位G缺失。G缺失终止了复制酶蛋白的翻译，并诱导了复制酶蛋白的截断。这些结果有助于对东北地区中国地区猪瘟病毒3型的分子流行病学和进化的认识。鉴定出一株复制酶蛋白截短的PCV3新毒株。
First identified in the United States in 2016, porcine circovirus type 3 (PCV3) is a newly emerging porcine circovirus exhibiting a wide range of clinical syndromes, which may be associated with the pathogenicity observed in pigs. The aim of this study was to identify and characterize the full genome sequence of PCV3 strains circulating in Northeast China. Herein, 105 lung samples isolated from sick pigs in Northeast China during 2018 were analyzed for PCV3. Using PCR, the total PCV3-positive rate was 33.3% (35/105), with rates of 17.8% (8/45), 66.7% (10/15), and 37.8% (17/45) in Heilongjiang, Jilin, and Liaoning province, respectively. Additionally, our findings showed that PCV3-positive samples had a high rate of co-infection with PCV2, PPV6, and PPV7. To study the evolution of the PCV3 in Northeast China, we sequenced the entire genome of 13 strains of PCV3. The results of phylogenetic analyses revealed that PCV3 could be divided into two clades, PCV3a and PCV3b. Interestingly, a G deletion at position 1072 was found in the 1999 nt genome of PCV3-CN2018LN-4 (MH277118). The G deletion terminated replicase protein translation and induced a truncated replicase protein. These results contribute to the understanding of PCV3 molecular epidemiology and evolution in Northeast China. A new strain of PCV3 with truncated replicase protein was identified.
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影响因子：
11.8
作者：
Li L;McGraw S;Zhu K;Leutenegger CM;Marks SL;Kubiski S;Gaffney P;Dela Cruz FN Jr;Wang C;Delwart E;Pesavento PA
通讯作者：
Pesavento PA
影响因子：
5.2
作者：
Li G;Wang H;Wang S;Xing G;Zhang C;Zhang W;Liu J;Zhang J;Su S;Zhou J
通讯作者：
Zhou J
影响因子：
2.6
作者：
Saha D;Lefebvre DJ;Ducatelle R;Doorsselaere JV;Nauwynck HJ
通讯作者：
Nauwynck HJ
影响因子：
4.3
作者：
Chen, G. H.;Mai, K. J.;Ma, J. Y.
通讯作者：
Ma, J. Y.
影响因子：
4.3
作者：
Fu, X.;Fang, B.;Zhang, G.
通讯作者：
Zhang, G.