Outcome of experimental porcine circovirus type 1 infections in mid-gestational porcine foetuses.
Outcome of experimental porcine circovirus type 1 infections in mid-gestational porcine foetuses.
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DOI：
10.1186/1746-6148-7-64
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发表时间：
2011-10-21
影响因子：
2.6
通讯作者：
Nauwynck HJ
中科院分区：
文献类型：
--
作者：
Saha D;Lefebvre DJ;Ducatelle R;Doorsselaere JV;Nauwynck HJ
猪圆环病毒1型（PCV1）已被描述为PK-15细胞系的非细胞病变污染物。几次实验性PCV1感染未能在猪中再现疾病。因此，PCV1通常被认为对猪无致病性。据我们所知，对猪胎儿感染PCV1的结果一无所知。本研究对此进行了检验。将3头母猪的9个胎儿在妊娠55天接种：3个接种PCV1细胞培养株ATCC-CCL33的104.3 TCID50, 3个接种PCV1野株3384的104.3 TCID50, 3个接种细胞培养基（模拟接种）。接种后21 d， 6例接种pcv1的胎儿和3例模拟接种的胎儿外观均正常。在接种CCL33的两个胎儿的肺部观察到以严重出血为特征的显微镜病变。在接种ccl33的胎儿的肺部发现高PCV1滴度（高达104.7 TCID50/g组织）。ccl33接种的胎儿其他器官和3384接种的胎儿所有器官病毒滴定均为阴性（< 101.7 TCID50/g组织）。在心脏、肺、脾脏、肝脏、胸腺和扁桃体中发现pcv1阳性细胞（接种ccl33的胎儿中高达121个细胞/10 mm2，接种3384的胎儿中高达13个细胞/10 mm2）。Rep的PCR和DNA测序分别从接种CCL33或3384的胎儿中恢复CCL33或3384序列。本研究表明，细胞培养的PCV1在55天胎龄时接种的猪胎肺中能够高效复制并产生病理。
Porcine circovirus type 1 (PCV1) has been described as a non-cytopathic contaminant of the PK-15 cell line. Several experimental infections with PCV1 failed to reproduce disease in pigs. Therefore, PCV1 is generally accepted as non-pathogenic to pigs. To our knowledge, nothing is known about the outcome of PCV1 infections in porcine foetuses. This was examined in the present study. Nine foetuses from three sows were inoculated at 55 days of gestation: three with 104.3 TCID50 of the PCV1 cell culture strain ATCC-CCL33, three with 104.3 TCID50 of the PCV1 field strain 3384 and three with cell culture medium (mock-inoculated). At 21 days post-inoculation, all 6 PCV1-inoculated and all 3 mock-inoculated foetuses had a normal external appearance. Microscopic lesions characterized by severe haemorrhages were observed in the lungs of two foetuses inoculated with CCL33. High PCV1 titres (up to 104.7 TCID50/g tissue) were found in the lungs of the CCL33-inoculated foetuses. All other organs of the CCL33-inoculated foetuses and all the organs of the 3384-inoculated foetuses were negative (< 101.7 TCID50/g tissue) by virus titration. PCV1-positive cells (up to 121 cells/10 mm2 in CCL33-inoculated foetuses and up to 13 cells/10 mm2 in 3384-inoculated foetuses) were found in the heart, lungs, spleen, liver, thymus and tonsils. PCR and DNA sequencing of Rep recovered CCL33 or 3384 sequences from CCL33- or 3384-inoculated foetuses, respectively. From this study, it can be concluded that cell culture PCV1 can replicate efficiently and produce pathology in the lungs of porcine foetuses inoculated at 55 days of foetal life.
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影响因子：
3.3
作者：
Saha, D.;Lefebvre, D. J.;Nauwynck, H. J.
通讯作者：
Nauwynck, H. J.
影响因子：
2.7
作者：
JOO, HS;DONALDSONWOOD, CR;JOHNSON, RH
通讯作者：
JOHNSON, RH
影响因子：
1.8
作者：
PESCOVITZ, MD;AASTED, B;SAALMUELLER, A
通讯作者：
SAALMUELLER, A
影响因子：
5.4
作者：
Fenaux, M;Opriessnig, T;Meng, XJ
通讯作者：
Meng, XJ
影响因子：
4.4
作者：
Costers S;Lefebvre DJ;Goddeeris B;Delputte PL;Nauwynck HJ
通讯作者：
Nauwynck HJ