Neuronal representation of conditioned taste in the basolateral amygdala of rats.
Neuronal representation of conditioned taste in the basolateral amygdala of rats.
复制标题
大鼠基底外侧杏仁核条件味觉的神经元表征。
DOI：
10.1016/j.nlm.2009.12.007
复制
发表时间：
2010-03
影响因子：
2.7
通讯作者：
Bernstein, Ilene L.
中科院分区：
文献类型：
--
作者：
Kim, Min Jung;Mizumori, Sheri J. Y.;Bernstein, Ilene L.
一旦动物体验到一种新的味道，然后内脏不适，它们就会发展出强大的学习能力和持久的味觉厌恶记忆。大量的文献支持这一假设，即基底外侧杏仁核（BLA）在这种条件性味觉厌恶（CTA）的获得和消退中起着关键作用。尽管有证据表明BLA在CTA训练中至关重要，但目前尚不清楚BLA神经活动如何代表条件性味觉。在这里，我们纳入了一个修改后的行为范式适合于单单位的研究，一个利用脉冲糖精和水输注通过口内插管的序列。条件反射后，我们研究了BLA单位的活动，而动物的经验，条件反射的味道（糖精）。味觉反应的行为测试证实，利用的训练程序产生可靠的收购和表达的厌恶整个测试会话。当糖精和水试验之间的神经活动进行了比较，一半的记录BLA单位（77/149）显示出不同的活动，根据类型的解决方案。条件组中76%的这些细胞（29/38）显示出受抑制的活性，而对照组中只有44%的味觉反应细胞（17/39）在糖精试验期间（相对于水试验）显示出受抑制的活性。此外，BLA单位的整体兴奋性增加，如条件反射后爆发活动特征的改变所示。在BLA活动的变化作为CTA的结果保持整个测试会话，与行为研究一致。目前的研究表明，由甜味引起的神经元活动作为CTA学习的结果而改变，并且整体变化可能与学习诱导的负面情绪有关。
Animals develop robust learning and long lasting taste aversion memory once they experience a new taste that is followed by visceral discomfort. A large body of literature has supported the hypothesis that basolateral amygdala (BLA) plays a critical role in the acquisition and extinction of such conditioned taste aversions (CTA). Despite the evidence that BLA is crucially engaged during CTA training, it is unclear how BLA neural activity represents the conditioned tastes. Here, we incorporated a modified behavioral paradigm suitable for single unit study, one which utilizes a sequence of pulsed saccharin and water infusion via intraoral cannulae. After conditioning, we investigated BLA unit activity while animals experience the conditioned taste (saccharin). Behavioral tests of taste reactivity confirmed that the utilized training procedure produced reliable acquisition and expression of the aversion throughout test sessions. When neural activity was compared between saccharin and water trials, half of the recorded BLA units (77/149) showed differential activity according to the types of solution. 76% of those cells (29/38) in the conditioned group showed suppressed activity, while only 44% of taste reactive cells (17/39) in controls showed suppressed activity during saccharin trials (relative to water trials). In addition, the overall excitability of BLA units was increased as shown by altered characteristics of burst activity after conditioning. The changes in BLA activity as a consequence of CTA were maintained throughout test sessions, consistent with the behavioral study. The current study suggests that the neuronal activity evoked by a sweet taste is altered as a consequence of CTA learning, and that the overall change might be related to the learning induced negative affect.
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DOI：
10.1073/pnas.0808996106
发表时间：
2008-12-30
影响因子：
11.1
作者：
Barot, Sabiha K.;Kyono, Yasuhiro;Bernstein, Ilene L.
通讯作者：
Bernstein, Ilene L.
影响因子：
2.5
作者：
Steriade, M;Timofeev, I;Grenier, F
通讯作者：
Grenier, F
影响因子：
2.5
作者：
YAMAMOTO, T;MATSUO, R;KITAMURA, R
通讯作者：
KITAMURA, R
影响因子：
1.9
作者：
Pratt, WE;Mizumori, SJY
通讯作者：
Mizumori, SJY
影响因子：
16.2
作者：
Harris, KD;Hirase, H;Buzsáki, G
通讯作者：
Buzsáki, G