A new method for chlorhexidine (CHX) determination: CHX release after application of differently concentrated CHX-containing preparations on artificial fissures.
A new method for chlorhexidine (CHX) determination: CHX release after application of differently concentrated CHX-containing preparations on artificial fissures.
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DOI：
10.1007/s00784-007-0166-4
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发表时间：
2008-09
影响因子：
3.4
通讯作者：
Attin R
中科院分区：
文献类型：
--
作者：
Attin T;Abouassi T;Becker K;Wiegand A;Roos M;Attin R
本研究的目的是(1)建立小容量洗必泰(CHX)的定量方法；(2)测定不同浓度的CHX制剂、清漆和用于人工裂隙的CHX凝胶的CHX释放。CHX的测定是在微板阅读器中进行的，使用的是聚苯乙烯孔。用还原后的微孔板的荧光强度进行CHX定量。为了验证该技术，计算了CHX浓度分级系列的批内和批间变异系数，并确定了定量下限(LLOQ)。另取50个牛牙釉质标本，随机分为5组(A-E，n = 10)，分别放置于蒸馏水中(7天)；A：CHX-清漆EC40；B：CHX-清漆CEVITEC；C：CHX-GELL氯六甲胺；D：阴性对照，不涂CHX；E：CHX-二乙酸酯标准品(E1，n = 5)或CHX-二葡萄糖酸盐(E2，n = 5)。每天刷牙，测量溶液中的CHX。该方法的批内和批间变异系数分别为-lt；10和-lt；20%，最低检出限为0.91-1.22nmol/孔。7天内CHX的累积释放量(平均 ± SD)为：EC40(217.2 ± 41.8nmol)，CHX-Gel(31.3 ± 8.5nmol)，Cervitec(18.6 ± 1.7nmol)。A-C组CHX释放量明显高于D组，且呈持续性释放，EC40在0~7d的释放量最高，氯化十六烷基三氯甲烷的释放量最低。新方法是小体积定量CHX的可靠工具。这两种测试的清漆都显示出比测试的CHX凝胶更长时间和更高的CHX从人工裂缝中的释放。
Aims of the study were (1) to establish a method for quantification of chlorhexidine (CHX) in small volumes and (2) to determine CHX release from differently concentrated CHX-containing preparations, varnishes, and a CHX gel applied on artificial fissures. CHX determination was conducted in a microplate reader using polystyrene wells. The reduced intensity of fluorescence of the microplates was used for CHX quantification. For verification of the technique, intra- and inter-assay coefficients of variation were calculated for graded series of CHX concentrations, and the lower limit of quantification (LLOQ) was determined. Additionally, artificial fissures were prepared in 50 bovine enamel samples, divided into five groups (A–E, n = 10) and stored in distilled water (7 days); A: CHX-varnish EC40; B: CHX-varnish Cervitec; C: CHX-gel Chlorhexamed; D: negative control, no CHX application; and E: CXH-diacetate standard (E1, n = 5) or CHX-digluconate (E2, n = 5) in the solution. The specimens were brushed daily, and CHX in the solution was measured. The method showed intra- and inter-assay coefficients of variation of <10 and <20%, respectively; LLOQ was 0.91–1.22 nmol/well. The cumulative CHX release (mean ± SD) during the 7 days was: EC40 (217.2 ± 41.8 nmol), CHX-gel (31.3 ± 8.5 nmol), Cervitec (18.6 ± 1.7 nmol). Groups A–C revealed a significantly higher CHX release than group D and a continuous CHX-release with the highest increase from day 0 to 7 for EC40 and the lowest for Chlorhexamed. The new method is a reliable tool to quantify CHX in small volumes. Both tested varnishes demonstrate prolonged and higher CHX release from artificial fissures than the CHX-gel tested.
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影响因子：
4.2
作者：
DEVRIES, J;RUBEN, J;ARENDS, J
通讯作者：
ARENDS, J
影响因子：
2.2
作者：
Delbem, ACB;Pedrini, D;Machado, TM
通讯作者：
Machado, TM
影响因子：
4.4
作者：
Joharji, RM;Adenubi, JO
通讯作者：
Adenubi, JO
DOI：
10.1016/s0378-4347(00)83482-9
发表时间：
1986-12-19
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
BROUGHAM, LR;CHENG, H;PITTMAN, KA
通讯作者：
PITTMAN, KA
影响因子：
3.4
作者：
Attin, Rengin;Ilse, Anika;Attin, Thomas
通讯作者：
Attin, Thomas