Cyclin-dependent kinase complexes in developing maize endosperm: evidence for differential expression and functional specialization.
Cyclin-dependent kinase complexes in developing maize endosperm: evidence for differential expression and functional specialization.
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DOI：
10.1007/s00425-013-1990-1
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发表时间：
2014-02
期刊：
影响因子：
4.3
通讯作者：
Larkins BA
中科院分区：
文献类型：
--
作者：
Dante RA;Sabelli PA;Nguyen HN;Leiva-Neto JT;Tao Y;Lowe KS;Hoerster GJ;Gordon-Kamm WJ;Jung R;Larkins BA
玉米（Zea mays L.）和相关谷物的胚乳发育包括细胞增殖阶段，随后是快速生长阶段和内复制阶段。人们对胚乳发育过程中细胞周期的调节知之甚少。我们已经表征了细胞周期蛋白依赖性激酶（CDK）复合物的各种亚基，即所有真核生物中的主要细胞周期调节因子。 A、B 和 D 型细胞周期蛋白以及 A 和 B 型细胞周期蛋白依赖性激酶的 RNA 和蛋白质表达谱进行了表征。确定了两种主要模式：一种在整个胚乳发育过程中表现出表达，另一种特征是随着核内复制的开始而急剧下调。细胞周期蛋白CYCB1;3和CYCD2;1蛋白分布在整个胚乳细胞的细胞质和细胞核中，而细胞周期蛋白CYCD5蛋白定位于外周细胞的细胞质中。 CDKB1;1 表达与细胞增殖密切相关。表达和细胞周期蛋白结合模式表明 CDKA;1 和 CDKA;3 至少部分冗余。与细胞周期蛋白 CYCA1 相关的激酶活性在发育有丝分裂阶段最高，而与 CYCB1;3、CYCD2;1 和 CYCD5 相关的激酶活性在有丝分裂到核内复制转变时达到峰值。 A、B 和 D 型细胞周期蛋白在内复制过程中比有丝分裂胚乳提取物更能抵抗蛋白酶体依赖性降解。这些结果表明，胚乳发育的特点是特定细胞周期蛋白和 CDK 的差异表达和活性，并表明核内复制与通过泛素-蛋白酶体途径减少的细胞周期蛋白水解有关。本文的在线版本 (doi:10.1007/s00425-013-1990-1) 包含补充材料，可供授权用户使用。
Endosperm development in maize (Zea mays L.) and related cereals comprises a cell proliferation stage followed by a period of rapid growth coupled to endoreduplication. Regulation of the cell cycle in developing endosperm is poorly understood. We have characterized various subunits of cyclin-dependent kinase (CDK) complexes, master cell cycle regulators in all eukaryotes. A-, B-, and D-type cyclins as well as A- and B-type cyclin-dependent kinases were characterized with respect to their RNA and protein expression profiles. Two main patterns were identified: one showing expression throughout endosperm development, and another characterized by a sharp down-regulation with the onset of endoreduplication. Cyclin CYCB1;3 and CYCD2;1 proteins were distributed in the cytoplasm and nucleus of cells throughout the endosperm, while cyclin CYCD5 protein was localized in the cytoplasm of peripheral cells. CDKB1;1 expression was strongly associated with cell proliferation. Expression and cyclin-binding patterns suggested that CDKA;1 and CDKA;3 are at least partially redundant. The kinase activity associated with the cyclin CYCA1 was highest during the mitotic stage of development, while that associated with CYCB1;3, CYCD2;1 and CYCD5 peaked at the mitosis-to-endoreduplication transition. A-, B- and D-type cyclins were more resistant to proteasome-dependent degradation in endoreduplicating than in mitotic endosperm extracts. These results indicated that endosperm development is characterized by differential expression and activity of specific cyclins and CDKs, and suggested that endoreduplication is associated with reduced cyclin proteolysis via the ubiquitin–proteasome pathway. The online version of this article (doi:10.1007/s00425-013-1990-1) contains supplementary material, which is available to authorized users.
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