Impacts of sleep on the characteristics of dental biofilm.
Impacts of sleep on the characteristics of dental biofilm.
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DOI：
10.1038/s41598-020-80541-5
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发表时间：
2021-01-08
影响因子：
4.6
通讯作者：
Ebisu S
中科院分区：
文献类型：
--
作者：
Sotozono M;Kuriki N;Asahi Y;Noiri Y;Hayashi M;Motooka D;Nakamura S;Machi H;Iida T;Ebisu S
牙齿表面的生物膜与口腔疾病如龋齿和牙周病有关。由于睡眠期间唾液中的细菌数量会迅速增加，因此建议睡前进行口腔护理以预防慢性口腔疾病。然而，牙齿生物膜的数量和质量的时间昼夜变化知之甚少。本研究旨在通过原位模型研究睡眠对牙齿生物膜数量和组成的影响。使用这种原位模型，使我们能够调查在口腔中形成的牙齿生物膜，并进行定量分析。受试者在早上或睡觉前开始戴口腔夹板，并在受试者开始戴口腔夹板后8，16和24小时收集生物膜样本;然后将这些样本用于各种实验。睡眠没有引起生物膜形成细菌数量的显着变化。然而，与牙周炎相关的属的相对丰度（即，梭杆菌属和普雷沃菌属）在觉醒后增加。总之，生物膜形成细菌的数量不受睡眠的影响，而专性厌氧菌的丰度在睡眠后增加。这项研究可能有助于确定有效的预防性口腔护理。
Dental biofilm present on the tooth surface is associated with oral diseases, such as dental caries and periodontal disease. Because bacterial numbers rapidly increase in saliva during sleep, oral care before sleeping is recommended for the prevention of chronic oral diseases. However, temporal circadian changes in the quantity and quality of dental biofilms are poorly understood. This study aimed to investigate the impacts of sleeping on dental biofilm amounts and compositions by using an in situ model. The use of this in situ model enabled us to investigate dental biofilm formed in the oral cavity and to perform a quantitative analysis. Subjects began wearing oral splints in the morning or before sleeping, and biofilm samples were collected at 8, 16, and 24 h after the subjects began wearing oral splints; these samples were then used in various experiments. No significant changes in the numbers of biofilm-forming bacteria were caused by sleep. However, the relative abundances of genera related to periodontitis (i.e., Fusobacterium and Prevotella) increased after awakening. In conclusion, the numbers of biofilm-forming bacteria were not affected by sleep, and the abundances of obligate anaerobes increased after sleep. This research may aid in defining efficacious preventive oral care.
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影响因子：
9.2
作者：
Hall MW;Singh N;Ng KF;Lam DK;Goldberg MB;Tenenbaum HC;Neufeld JD;G Beiko R;Senadheera DB
通讯作者：
Senadheera DB
影响因子：
4.6
作者：
Sotozono M;Kuriki N;Asahi Y;Noiri Y;Hayashi M;Motooka D;Nakamura S;Machi H;Iida T;Ebisu S
通讯作者：
Ebisu S
影响因子：
3.7
作者：
Yamamoto R;Noiri Y;Yamaguchi M;Asahi Y;Maezono H;Kuboniwa M;Hayashi M;Ebisu S
通讯作者：
Ebisu S
影响因子：
4.3
作者：
LANG, NP;CUMMING, BR;LOE, H
通讯作者：
LOE, H
影响因子：
3.7
作者：
Hajishengallis G;Lamont RJ
通讯作者：
Lamont RJ