Evaluation of Bi-Layer Silk Fibroin Grafts for Penile Tunica Albuginea Repair in a Rabbit Corporoplasty Model.
Evaluation of Bi-Layer Silk Fibroin Grafts for Penile Tunica Albuginea Repair in a Rabbit Corporoplasty Model.
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DOI：
10.3389/fbioe.2021.791119
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发表时间：
2021
影响因子：
5.7
通讯作者：
Mauney JR
中科院分区：
文献类型：
--
作者：
Gundogdu G;Okhunov Z;Starek S;Veneri F;Orabi H;Holzman SA;Sullivan MP;Khoury AE;Mauney JR
自体组织移植在Peyronie病和先天性脊索炎白膜修复中的应用往往受到组织可获得性和供区发病率的限制，因此需要新的生物材料选择。在本研究中，研究了双层丝素蛋白(BLSF)支架材料在兔骨盆成形术模型中对白膜功能组织再生的支持作用。18只成年雄性新西兰白兔随机分为非手术对照组(NSC，N=3)、BLSF移植组(N=5)、脱细胞小肠黏膜下基质(SIS)组(N=5)和自体阴道膜(TV)瓣组(N=5)。终点评估包括海绵体造影术、海绵体测量、组织学、免疫组织化学和组织形态计量学评估。罂粟碱诱导勃起后的最大暂时内压(ICP)在所有组之间都是相似的。使用BLSF支架或电视瓣修复的兔子中，80%的兔实现了完全刚性勃起，相比之下，SIS重建动物的这一比例为40%。60%的BLSF兔保持了5分钟的峰值勃起，而SIS和TV瓣重建的兔只有20%。在最大颅内压时，TV组的移植物穿孔发生率为60%，而BLSF组为20%。由SIS和BLSF支架支撑的新生组织由类似于神经干细胞的I型胶原和弹性蛋白纤维组成。SIS和TV瓣显示，相对于NSC，小体纤维化水平显著升高，而表达收缩蛋白的小体平滑肌细胞相应减少。BLSF生物材料代表了骨盆成形术的新兴平台，与TV瓣和SIS基质相比，可产生更好的功能和组织学效果，用于白膜修复。
The use of autologous tissue grafts for tunica albuginea repair in Peyronie’s disease and congenital chordee is often restricted by limited tissue availability and donor site morbidity, therefore new biomaterial options are needed. In this study, bi-layer silk fibroin (BLSF) scaffolds were investigated to support functional tissue regeneration of tunica albuginea in a rabbit corporoplasty model. Eighteen adult male, New Zealand white rabbits were randomized to nonsurgical controls (NSC, N = 3), or subjected to corporoplasty with BLSF grafts (N = 5); decellularized small intestinal submucosa (SIS) matrices (N = 5); or autologous tunica vaginalis (TV) flaps (N = 5). End-point evaluations were cavernosography, cavernosometry, histological, immunohistochemical, and histomorphometric assessments. Maximum intracorporal pressures (ICP) following papaverine-induced erection were similar between all groups. Eighty percent of rabbits repaired with BLSF scaffolds or TV flaps achieved full rigid erections, compared to 40% of SIS reconstructed animals. Five-minute peak erections were maintained in 60% of BLSF rabbits, compared to 20% of SIS and TV flap reconstructed rabbits. Graft perforation occurred in 60% of TV group at maximum ICP compared to 20% of BLSF cohort. Neotissues supported by SIS and BLSF scaffolds were composed of collagen type I and elastin fibers similar to NSC. SIS and TV flaps showed significantly elevated levels of corporal fibrosis relative to NSC with a corresponding decrease in corporal smooth muscle cells expressing contractile proteins. BLSF biomaterials represent emerging platforms for corporoplasty and produce superior functional and histological outcomes in comparison to TV flaps and SIS matrices for tunica albuginea repair.
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影响因子：
3.7
作者：
Chung YG;Tu D;Franck D;Gil ES;Algarrahi K;Adam RM;Kaplan DL;Estrada CR Jr;Mauney JR
通讯作者：
Mauney JR
影响因子：
3.5
作者：
Chung, Eric;Clendinning, Eric;Brock, Gerald
通讯作者：
Brock, Gerald
影响因子：
6.6
作者：
Chun, JL;McGregor, A;Carson, CC
通讯作者：
Carson, CC
影响因子：
2.1
作者：
Knoll, LD
通讯作者：
Knoll, LD
影响因子：
6.6
作者：
Ritchey, ML;Ribbeck, M
通讯作者：
Ribbeck, M