Transcriptomes in healthy and diseased gingival tissues.
Transcriptomes in healthy and diseased gingival tissues.
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DOI：
10.1902/jop.2008.080139
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发表时间：
2008-11
影响因子：
4.3
通讯作者：
Papapanou PN
中科院分区：
文献类型：
--
作者：
Demmer RT;Behle JH;Wolf DL;Handfield M;Kebschull M;Celenti R;Pavlidis P;Papapanou PN
临床和影像学检测是诊断牙周炎的金标准，但对于该疾病的发病机制提供的信息很少。我们假设对健康和患病牙龈组织之间的基因表达特征进行比较，将为牙周炎的病理生物学提供新的见解，并为未来的研究设计提供依据。
90名全身健康的非吸烟者患有中度至重度牙周炎（63人患有慢性牙周炎，27人患有侵袭性牙周炎），每人提供了≥2个“患病”的邻间乳头[探诊出血（BoP），牙周袋深度（PD）≥4mm，附着丧失（AL）≥3mm]以及一个“健康”乳头（如果有的话，无BoP，PD≤4mm且AL≤2mm）。提取RNA，进行扩增、反转录、标记，并与Affymetrix U133Plus2.0芯片杂交。使用标准的混合效应线性模型方法对247个个体组织样本（183个来自患病部位，64个来自健康部位）进行差异表达检测，将患者效应视为具有正态分布的随机效应，将牙龈组织状态视为两级固定效应。基因本体分析将表达模式归纳为具有生物学相关性的类别。
转录组分析显示，在对多重比较进行校正后，共有12744个探针组存在差异表达（p<9.15×10⁻⁷）。其中，与健康组织相比，在患病组织中有5295个探针组上调，7449个探针组下调。基因本体分析确定了61个差异表达组（校正后p<0.05），包括细胞凋亡、抗菌体液反应、抗原呈递、代谢过程的调节、信号转导和血管生成。
牙龈组织转录组为进一步以假设为导向的牙周炎病理生物学研究提供了一种有价值的科学工具。
Clinical and radiographic measures are gold standards for diagnosing periodontitis but offer little information regarding the pathogenesis of the disease. We hypothesized that a comparison of gene expression signatures between healthy and diseased gingival tissues would provide novel insights in the pathobiology of periodontitis, and would inform the design of future studies. Ninety systemically healthy non-smokers with moderate to advanced periodontitis (63 with chronic and 27 with aggressive periodontitis) each contributed with ≥2 “diseased” interproximal papillae [with bleeding on probing (BoP), pocket depth (PD) ≥4mm, and attachment loss (AL) ≥3mm)] and a “healthy” papilla, if available (no BoP, PD ≤4mm and AL ≤2mm). RNA was extracted, amplified, reverse-transcribed, labeled, and hybridized with AffymetrixU133Plus2.0 arrays. Differential expression was assayed in 247 individual tissue samples (183 from diseased and 64 from healthy sites) using a standard mixed-effects linear model approach, with patient effects considered random with a normal distribution, and gingival tissue status considered a two-level fixed effect. Gene ontology analysis summarized the expression patterns into biologically relevant categories. Transcriptome analysis revealed that a total of 12,744 probe sets were differentially expressed after adjusting for multiple comparisons (p<9.15×10-7). Of those, 5,295 were up-regulated and 7,449 down-regulated in disease when compared to health. Gene ontology analysis identified 61 differentially expressed groups (adjusted p<0.05) including apoptosis, antimicrobial humoral response, antigen presentation, regulation of metabolic processes, signal transduction, and angiogenesis. Gingival tissue transcriptomes provide a valuable scientific tool for further hypothesis-driven studies of the pathobiology of periodontitis.
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影响因子：
14.9
作者：
Khatri P;Voichita C;Kattan K;Ansari N;Khatri A;Georgescu C;Tarca AL;Draghici S
通讯作者：
Draghici S
影响因子：
4.3
作者：
Kubota, Takehiko;Itagaki, Manarni;Yoshie, Hiromasa
通讯作者：
Yoshie, Hiromasa
影响因子：
3.4
作者：
Handfield, M;Mans, JJ;Lamont, RJ
通讯作者：
Lamont, RJ
DOI：
10.1016/s0736-5748(02)00041-2
发表时间：
2002-06-01
影响因子：
1.8
作者：
Haslett, JN;Kunkel, LM
通讯作者：
Kunkel, LM
影响因子：
6.7
作者：
LISTGARTEN, MA;HELLDEN, L
通讯作者：
HELLDEN, L