Herbicide treatments alter carotenoid profiles for 14C tracer production from tomato ( Solanum lycopersicum cv. VFNT cherry) cell cultures.
Herbicide treatments alter carotenoid profiles for 14C tracer production from tomato ( Solanum lycopersicum cv. VFNT cherry) cell cultures.
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DOI：
10.1021/jf803905d
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发表时间：
2009-06-10
影响因子：
6.1
通讯作者：
Erdman JW Jr
中科院分区：
文献类型：
--
作者：
Engelmann NJ;Rogers RB;Lila MA;Erdman JW Jr
由于缺乏商品化的放射性标记番茄红素(LyC)、植烯(PE)和植物荧烯(PF)，对类胡萝卜素生物活性机制的研究进展有限。番茄(Solanum Lycopsicum cv.)细胞培养产生[14C]-PE和PF，但产量相对较低。为了提高类胡萝卜素的产量，在培养过程中分别使用两种漂白除草剂2-(4-氯苯硫基)三乙胺和去氟草松，以产生不同比例的PE、PF和LYC。用这两种除草剂处理后，所有三种类胡萝卜素的产量都达到最佳。随后，将培养物在含蔗糖的[14C]培养基中孵育，产生标记的LyC、PE和PF。在14天培养周期的第一天加入[14C]蔗糖到去甲氟拉松处理的培养物中，与在第七天添加蔗糖相比，标记效率略有增加。改进的培养条件有效地为未来的细胞培养和动物代谢跟踪研究提供了充足的14C-胡萝卜素。
Progress in learning about underlying carotenoid bioactivity mechanisms has been limited due to lack of commercially available radiolabeled lycopene (LYC), phytoene (PE), and phytofluene (PF). Tomato (Solanum lycopersicum cv. VFNT cherry) cell cultures have been treated to produce [14C]-PE and PF but with relatively low yields. To increase carotenoid production, two bleaching herbicides were administered during the culture incubation, 2-(4-chlorophenyl-thio) triethylamine and norflurazon separately or in combination to produce varying ratios of PE, PF, and LYC. Treatment with both herbicides resulted in optimal production of all three carotenoids. Subsequently, cultures were incubated in [14C] sucrose-containing media to produce labeled LYC, PE, and PF. Adding [14C] sucrose on day 1 of the 14-d culture incubation cycle to norflurazon-treated cultures led to a small increase in labeling efficiency compared to adding it on day 7. Improved culture conditions efficiently provided sufficient 14C-carotenoids for future cell culture and animal metabolic tracking studies.
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影响因子：
6.1
作者：
Lu, Chi-Hua;Engelmann, Nancy J.;Lila, Mary Ann;Erdman, John W., Jr.
通讯作者：
Erdman, John W., Jr.
影响因子：
3.9
作者：
Al-Babili, S;Hartung, W;Beyer, P
通讯作者：
Beyer, P
影响因子：
6.1
作者：
Ishida, BK;Mahoney, NE;Ling, LC
通讯作者：
Ling, LC
影响因子：
7.1
作者：
Paetau, I;Khachik, F;Clevidence, BA
通讯作者：
Clevidence, BA
影响因子：
4.7
作者：
Windhovel, U;Sandmann, G;Boger, P
通讯作者：
Boger, P