Phenotypic Plasticity, Epigenetic or Genetic Modifications in Relation to the Duration of Cd-Exposure within a Microevolution Time Range in the Beet Armyworm.
Phenotypic Plasticity, Epigenetic or Genetic Modifications in Relation to the Duration of Cd-Exposure within a Microevolution Time Range in the Beet Armyworm.
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DOI：
10.1371/journal.pone.0167371
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Zawisza-Raszka A
中科院分区：
文献类型：
--
作者：
Augustyniak M;Płachetka-Bożek A;Kafel A;Babczyńska A;Tarnawska M;Janiak A;Loba A;Dziewięcka M;Karpeta-Kaczmarek J;Zawisza-Raszka A
如果害虫栖息在金属污染或经常使用农药的田地里，就会发生抗性个体的自然选择。这可能给人类、农业以及许多国家的经济带来严重问题。在这项研究中，假设多代（120代）暴露于镉的甜菜夜蛾种群可能是一个选择因素，朝着更有效的DNA保护进行了验证。用H2O2（DNA损伤剂）或PBS（参考）处理来自两种培养菌株（对照和Cd暴露）的个体的血细胞。在处理后立即和5、15和30分钟使用彗星试验评估DNA损伤水平。与H2O2接触的直接结果是，来自两个品系的昆虫的血细胞中的DNA损伤水平显著增加。然而，在镉暴露个体的细胞中，DNA损伤水平随着时间的推移而下降，而在对照昆虫的细胞中，它保持在相同的水平，没有修复的证据。这些结果表明，有效的防御机制可能存在于长期接触镉的昆虫细胞。一些进化和权衡方面的现象进行了讨论。在更广泛的背景下，将实验室获得的结果与实地研究进行比较可能有助于了解生物体抗性的基本机制。总之，在镉品系昆虫中观察到的DNA损伤修复的高潜力可能证实了这样的假设，即多代暴露于该金属可能有助于选择对氧化应激具有更广泛耐受性的昆虫。然而，我们的调查多态性AFLP没有发现两个主要的昆虫品系之间的差异。
In the case of the pests inhabiting metal polluted or fields where the use of pesticides is common, a natural selection of resistant individuals can occur. This may pose serious problems for humans, agriculture, as well as the economies of many countries. In this study, the hypothesis that multigenerational (120 generations) exposure to cadmium of a beet armyworm population could be a selecting factor toward a more efficient DNA protection was verified. The hemocytes of individuals from two culture strains (control and Cd-exposed) were treated with H2O2 (a DNA-damaging agent) or PBS (reference). The level of DNA damage was assessed using the Comet assay immediately and 5, 15 and 30 min. after the treatment. The immediate result of the contact with H2O2 was that the level of DNA damage in the hemocytes of the insects from both strains increased significantly. However, in the cells of the Cd-exposed individuals, the level of DNA damage decreased over time, while in the cells from the control insects it remained at the same level with no evidence of repair. These results suggest that efficient defense mechanisms may exist in the cells of insects that have prolonged contact with cadmium. Some evolutionary and trade-off aspects of the phenomenon are discussed. In a wider context, comparing the results obtained in the laboratory with field studies may be beneficial for understanding basic mechanisms of the resistance of an organism. To summarize, the high potential for the repair of DNA damage that was observed in the insects from the cadmium strain may confirm the hypothesis that multigenerational exposure to that metal may possibly contribute to the selection of insects that have a wider tolerance to oxidative stress. However, our investigations of polymorphism using AFLP did not reveal differences between the two main insect strains.
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DOI：
10.1016/j.mrgentox.2010.10.008
发表时间：
2011-01-10
影响因子：
1.9
作者：
Carmona, Erico R.;Creus, Amadeu;Marcos, R.
通讯作者：
Marcos, R.
影响因子：
6.8
作者：
Kafel, Alina;Nowak, Aleksandra;Swiergosz-Kowalewska, Renata
通讯作者：
Swiergosz-Kowalewska, Renata
影响因子：
1.3
作者：
Kojic, Danijela;Spasojevic, Ivan;Spasic, Mihajlo B.
通讯作者：
Spasic, Mihajlo B.
影响因子：
2.8
作者：
Carmona, Erico R.;Guecheva, Temenouga N.;Marcos, Ricardo
通讯作者：
Marcos, Ricardo
DOI：
10.1016/j.mrgentox.2011.05.008
发表时间：
2011-09-18
影响因子：
1.9
作者：
Carmona, Erico R.;Creus, Amadeu;Marcos, Ricard
通讯作者：
Marcos, Ricard