Cross Brain-Gut Analysis Highlighted Hub Genes and LncRNA Networks Differentially Modified During Leucine Consumption and Endurance Exercise in Mice with Depression-Like Behaviors.
Cross Brain-Gut Analysis Highlighted Hub Genes and LncRNA Networks Differentially Modified During Leucine Consumption and Endurance Exercise in Mice with Depression-Like Behaviors.
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交叉脑肠道分析突出显示了具有抑郁样行为的小鼠在亮氨酸消耗和耐力运动期间差异修饰的Hub基因和LncRNA网络。
DOI：
10.1007/s12035-022-02835-1
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发表时间：
2022-07
影响因子：
5.1
通讯作者：
Hajibabaie, Fatemeh
中科院分区：
文献类型：
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作者：
Abedpoor, Navid;Taghian, Farzaneh;Hajibabaie, Fatemeh
抑郁症是一种常见的情绪障碍，可能会损害脑肠轴。在这项研究中，我们将30只小鼠分为五组：未经治疗的小鼠、有抑郁样行为的小鼠、有抑郁样行为的小鼠、有抑郁样行为的小鼠、有运动训练的小鼠、有抑郁样行为的小鼠、有运动训练和有消耗亮氨酸的小鼠。通过人工智能生物学分析，我们发现海马区和回肠组织中的lncRNAs谱和Kdr/Vegfα/Pten/Bdnf相互作用网络等介质可能是脑肠轴的决定性分子。此外，基于药效团模型和分子对接结果，KDR作为网络中的主要切割点蛋白被确定为改善重度抑郁症的药物方法。此外，我们发现在抑郁样行为小鼠中，Pten mRNA和蛋白水平升高，Vegfα/Kdr/Bdnf mRNA和Kdr蛋白水平降低。此外，运动和亮氨酸改善了具有抑郁样行为的小鼠的脑肠轴。运动和亮氨酸调节抑郁样行为小鼠海马和回肠的lncRNAs网络。我们建议lncRNAs谱可以被视为诊断和预后的生物标志物，运动+亮氨酸可能是改善抑郁症的实用方法。
Depression is a frequent mood disorder that might impair the brain-gut axis. In this study, we divided 30 mice into five groups: untreated mice, mice with depression-like behaviors, mice with depression-like behaviors treated with consumed leucine, mice with depression-like behaviors treated with exercise training, mice with depression-like behaviors treated with exercise training along with consumed leucine. According to artificial intelligence biological analysis, we found some mediators such as lncRNAs profile and Kdr/Vegfα/Pten/Bdnf interactions network in the hippocampus region and ileum tissue which could be decisive molecules in the brain-gut axis. Moreover, KDR as a principal cutpoint protein in the network was identified as the pharmaceutical approach for major depressive ameliorating based on pharmacophore modeling and molecular docking outcomes. Furthermore, we indicated that the mRNA and protein level of the Pten enhanced and Vegfα/Kdr/Bdnf mRNAs, as well as the protein level of KDR, decreased in mice with depression-like behaviors. Moreover, exercise and leucine ameliorated the brain-gut axis in mice with depression-like behaviors. Exercise and leucine regulated the lncRNAs network in the hippocampus and ileum of mice with depression-like behaviors. We suggest that the lncRNAs profiles could be considered as diagnosis and prognosis biomarkers, and exercise + leucine might be a practical approach to improve depression.
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影响因子：
4.5
作者：
Abedpoor N;Taghian F;Ghaedi K;Niktab I;Safaeinejad Z;Rabiee F;Tanhaei S;Nasr-Esfahani MH
通讯作者：
Nasr-Esfahani MH
影响因子：
4.1
作者：
Firk, Christine;Markus, C. Rob
通讯作者：
Markus, C. Rob
影响因子：
4.6
作者：
Daina A;Michielin O;Zoete V
通讯作者：
Zoete V
影响因子：
14.9
作者：
Burley SK;Bhikadiya C;Bi C;Bittrich S;Chen L;Crichlow GV;Christie CH;Dalenberg K;Di Costanzo L;Duarte JM;Dutta S;Feng Z;Ganesan S;Goodsell DS;Ghosh S;Green RK;Guranović V;Guzenko D;Hudson BP;Lawson CL;Liang Y;Lowe R;Namkoong H;Peisach E;Persikova I;Randle C;Rose A;Rose Y;Sali A;Segura J;Sekharan M;Shao C;Tao YP;Voigt M;Westbrook JD;Young JY;Zardecki C;Zhuravleva M
通讯作者：
Zhuravleva M
RNA在意外的地方：长的非编码RNA在不同的细胞环境中起作用。
DOI：
10.1038/nrm3679
发表时间：
2013-11
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
Geisler S;Coller J
通讯作者：
Coller J