Chemokine-like factor 1 (CLFK1) is over-expressed in patients with atopic dermatitis.
Chemokine-like factor 1 (CLFK1) is over-expressed in patients with atopic dermatitis.
复制标题
趋化因子样因子 1 (CLFK1) 在特应性皮炎患者中过度表达。
DOI：
10.7150/ijbs.6291
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发表时间：
2013
影响因子：
9.2
通讯作者：
Qian G
中科院分区：
文献类型：
--
作者：
Yang GY;Chen X;Sun YC;Ma CL;Qian G
背景：人趋化因子样因子1 （CKLF1）是最近发现的一种趋化因子，在免疫介导的疾病中具有广泛的生物学功能。它在Th2淋巴细胞上高度表达，是人CCR4的功能性配体。CKLF1在白细胞的募集和激活中起主要作用，在过敏性疾病的发病机制中起重要作用。本研究旨在确定CKLF1在特应性皮炎（AD）患者皮肤和血清中的表达。方法：采用免疫组织化学和酶联免疫吸附法分析皮肤病变组织中CKLF1蛋白的表达。Real-time PCR检测皮肤病变组织中CKLF1 mRNA的表达。ELISA法检测各组血清CKLF1、IgE、eotaxin、IL-4、IL-5、IL-13水平。结果：AD患者皮肤组织病理学改变表现为局部炎症，表皮增厚，炎性细胞明显浸润。免疫组化结果显示，CKLF1染色阳性细胞位于表皮和真皮层，且AD患者的CKLF1表达明显高于正常对照。AD患者的CKLF1 mRNA表达明显高于健康对照组。AD患者血清CKLF1和IgE水平显著升高，血清IL-4、IL-5、IL-13和eotaxin水平显著升高。结论：CKLF1蛋白和mRNA水平在AD患者皮损中均过表达，且血清中CKLF1水平升高，提示CKLF1可能在特应性皮炎的发生发展中发挥重要作用。
Background: Human chemokine-like factor 1 (CKLF1), a recently discovered chemokine, has a broad spectrum of biological functions in immune-mediated diseases. It is highly expressed on Th2 lymphocytes and is a functional ligand for human CCR4. CKLF1 has a major role in the recruitment and activation of leucocytes, which plays an important role in the pathogenesis of allergic diseases. The present study was designed to determine the expression of CKLF1 in skin and serum in patients with atopic dermatitis (AD). Methods: The CKLF1 protein expression in skin lesion was analyzed by immunohistochemistry and ELISA. The mRNA expression of CKLF1 in skin lesion was detected by Real-time PCR. The serum levels of CKLF1, IgE, eotaxin, IL-4, IL-5, and IL-13 were measured by ELISA. Results: Histopathological changes in the skin of AD patients showed local inflammation with epidermal thickening and significant inflammatory cellular infiltration. Immunohistochemistry results demonstrated that CKLF1-staining positive cells were located in the epidermal and dermis, and that the CKLF1 expression in AD patients was significantly higher than that in normal control. The CKLF1 mRNA expression in AD patients was significantly higher than that in healthy controls. Serum CKLF1 and IgE levels were significantly increased in AD patients, as were the serum levels of IL-4, IL-5, IL-13 and eotaxin. Conclusions: Both CKLF1 protien and mRNA levels are overexpressed in the skin lesion of AD patients, along with an increase in serum CKLF1 level, indicating that CKLF1 may play an important role in the development of atopic dermatitis.
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影响因子：
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