A hybrid biomimetic scaffold composed of electrospun polycaprolactone nanofibers and self-assembled peptide amphiphile nanofibers.
A hybrid biomimetic scaffold composed of electrospun polycaprolactone nanofibers and self-assembled peptide amphiphile nanofibers.
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DOI：
10.1088/1758-5082/1/2/025001
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发表时间：
2009-06
期刊：
影响因子：
9
通讯作者：
Jun HW
中科院分区：
文献类型：
--
作者：
Tambralli A;Blakeney B;Anderson J;Kushwaha M;Andukuri A;Dean D;Jun HW
纳米纤维静电纺丝聚（ε-己内酯）（ePCL）支架具有固有的结构优势，但缺乏生物活性限制了其在生物医学应用中的有用性。因此，在这里，我们报告的混合，纳米结构，细胞外基质（ECM）模拟支架的ePCL纳米纤维和自组装肽两亲物（PA）纳米纤维的组合的发展。PA具有ECM模拟特征，包括细胞粘附配体（RGDS）和基质金属蛋白酶-2（MMP-2）介导的可降解位点。TEM成像验证了使用溶剂蒸发方法成功地将PA自组装成纳米纤维（直径为8 - 10 nm）。然后使用这种蒸发涂覆方法将PA成功地涂覆到ePCL纳米纤维（直径为300 - 400 nm）上，以开发混合的生物活性支架。SEM表征表明PA涂层不干扰多孔ePCL纳米网络。将人骨髓间充质干细胞（hMSCs）接种到杂化支架上，以评估其生物活性。与涂覆有PA-S（无细胞粘附配体）的ePCL纳米纤维和未涂覆的ePCL纳米纤维相比，在涂覆有PA-RGDS的ePCL纳米纤维上观察到hMSC的显著更大的附着和铺展。总的来说，这种新的策略提出了一种新的解决方案，以克服电纺支架目前的生物活性挑战，并结合了ePCL纳米纤维和自组装PA纳米纤维的独特特性，以提供ECM模拟环境。这具有很大的潜力，可应用于许多不同的静电纺丝支架的各种生物医学应用。
Nanofibrous electrospun poly (ε-caprolactone) (ePCL) scaffolds have inherent structural advantages, but lack of bioactivity has limited their usefulness in biomedical applications. Thus, here we report the development of a hybrid, nanostructured, extracellular matrix (ECM) mimicking scaffold by a combination of ePCL nanofibers and self-assembled peptide amphiphile (PA) nanofibers. The PAs have ECM mimicking characteristics including a cell adhesive ligand (RGDS) and matrix metalloproteinase-2 (MMP-2) mediated degradable sites. TEM imaging verified successful PA self-assembly into nanofibers (diameters of 8 – 10 nm) using a solvent evaporation method. This evaporation coating method was then used to successfully coat PAs onto ePCL nanofibers (diameters of 300 – 400 nm), to develop the hybrid, bioactive scaffolds. SEM characterization showed that the PA coatings did not interfere with the porous ePCL nanofiber network. Human mesenchymal stem cells (hMSCs) were seeded onto the hybrid scaffolds to evaluate their bioactivity. Significantly greater attachment and spreading of hMSCs were observed on ePCL nanofibers coated with PA-RGDS as compared to ePCL nanofibers coated with PA-S (no cell adhesive ligand) and uncoated ePCL nanofibers. Overall, this novel strategy presents a new solution to overcome the current bioactivity challenges of electrospun scaffolds and combines the unique characteristics of ePCL nanofibers and self-assembled PA nanofibers to provide an ECM mimicking environment. This has great potential to be applied to many different electrospun scaffolds for various biomedical applications.
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影响因子：
14
作者：
Ma, ZW;Kotaki, M;Ramakrishna, S
通讯作者：
Ramakrishna, S
影响因子：
14
作者：
Li, WJ;Tuli, R;Tuan, RS
通讯作者：
Tuan, RS
影响因子：
9.7
作者：
Li, Wan-Ju;Cooper, James A., Jr.;Tuan, Rocky S.
通讯作者：
Tuan, Rocky S.
影响因子：
14
作者：
Buttafoco, L;Kolkman, NG;Feijen, J
通讯作者：
Feijen, J
DOI：
10.1163/156856204322752246
发表时间：
2004-01-01
影响因子：
3.6
作者：
Jun, HW;West, J
通讯作者：
West, J