Early fixation of cobalt-chromium based alloy surgical implants to bone using a tissue-engineering approach.
Early fixation of cobalt-chromium based alloy surgical implants to bone using a tissue-engineering approach.
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DOI：
10.3390/ijms13055528
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发表时间：
2012
影响因子：
5.6
通讯作者：
Tanaka Y
中科院分区：
文献类型：
--
作者：
Ogawa M;Tohma Y;Ohgushi H;Takakura Y;Tanaka Y
为了建立证明金属种植体与骨早期固定的方法，将钴铬（CoCr）基合金种植体表面的一侧播种兔骨髓间充质细胞，另一侧不播种。间充质细胞在抗坏血酸、β-甘油磷酸酯和地塞米松的作用下进一步培养，导致种植体表面出现成骨细胞和骨基质。因此，我们成功地在CoCr种植体的一侧生成了组织工程骨。然后将CoCr植入兔骨缺损。植入3周后进行力学试验评价、未钙化组织学切片及电镜分析。组织工程表面的组织学和电镜图像显示大量的新骨形成。然而，没有细胞播种的一侧很难检测到新形成的骨组织。在力学试验中，组织工程表面和非细胞播种表面的平均拔出力分别为77.15 N和44.94 N。这些发现表明，组织工程CoCr表面的早期骨固定仅在植入后三周。
To establish the methods of demonstrating early fixation of metal implants to bone, one side of a Cobalt-Chromium (CoCr) based alloy implant surface was seeded with rabbit marrow mesenchymal cells and the other side was left unseeded. The mesenchymal cells were further cultured in the presence of ascorbic acid, β-glycerophosphate and dexamethasone, resulting in the appearance of osteoblasts and bone matrix on the implant surface. Thus, we succeeded in generating tissue-engineered bone on one side of the CoCr implant. The CoCr implants were then implanted in rabbit bone defects. Three weeks after the implantation, evaluations of mechanical test, undecalcified histological section and electron microscope analysis were performed. Histological and electron microscope images of the tissue engineered surface exhibited abundant new bone formation. However, newly formed bone tissue was difficult to detect on the side without cell seeding. In the mechanical test, the mean values of pull-out forces were 77.15 N and 44.94 N for the tissue-engineered and non-cell-seeded surfaces, respectively. These findings indicate early bone fixation of the tissue-engineered CoCr surface just three weeks after implantation.
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DOI：
10.1007/s10856-011-4253-3
发表时间：
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影响因子：
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DOI：
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发表时间：
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期刊：
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影响因子：
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DOI：
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发表时间：
2002-06-05
期刊：
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作者：
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