Increased expression of type-1 fimbriae by nonpathogenic Escherichia coli 83972 results in an increased capacity for catheter adherence and bacterial interference.
Increased expression of type-1 fimbriae by nonpathogenic Escherichia coli 83972 results in an increased capacity for catheter adherence and bacterial interference.
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DOI：
10.1086/591093
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发表时间：
2008-09-15
期刊：
影响因子：
--
通讯作者：
Darouiche RO
中科院分区：
文献类型：
--
作者：
Trautner BW;Cevallos ME;Li H;Riosa S;Hull RA;Hull SI;Tweardy DJ;Darouiche RO
在体外，无毒大肠埃希菌83972的尿管定植会阻碍各种泌尿系病原菌随后的尿管定植。然而，大肠杆菌83972对硅胶导尿管材料的粘附率较低，可能是因为编码1型粘附剂菌毛的fim操纵子不完整。我们假设，改善大肠杆菌83972的导管粘附性将改善其细菌干扰性能。我们用fim+质粒转化野生型大肠杆菌83972，获得了粘附性突变体。使用超声试验研究了尿管的粘附性和防止致尿性大肠埃希氏菌定植导尿管的能力。添加单拷贝fim+质粒可使尿管粘附性增加10倍，添加18拷贝fim+质粒可使粘附性增加100倍。与野生型亲本菌株相比，粘附性更强的18拷贝fim+质粒菌株在阻止致病性大肠杆菌导管定植方面更有效。ΔFIM和FIM+E.Coli 83972均不能黏附于脱落的尿路上皮细胞。我们的结果表明，改善导尿管粘附性可以增强良性大肠杆菌83972的细菌干扰能力。增加1型菌毛的表达可能会增强细菌的干扰，而不会对大肠杆菌83972产生毒力。
In vitro, urinary catheter colonization by avirulent Escherichia coli 83972 impedes subsequent catheter colonization by a variety of uropathogenic organisms. However, E. coli 83972 shows a low efficacy of adherence to silicone urinary catheter material, possibly because the fim operon encoding adhesive type 1 fimbriae is incomplete. We hypothesized that improving the catheter adherence of E. coli 83972 would improve its bacterial interference properties. We created adhesive mutants by transforming wild-type E. coli 83972 with fim+ plasmids. Adherence to urinary catheters and ability to prevent uropathogenic E. coli from colonizing urinary catheters were studied by use of a sonication assay. The addition of a single-copy fim+ plasmid increased adherence to urinary catheters 10-fold, and addition of an 18-copy fim+ plasmid increased adherence 100-fold. The more adherent 18-copy fim+ plasmid strain was more effective at blocking catheter colonization by pathogenic E. coli than was the wild-type parental strain. Neither Δfim nor fim+ E. coli 83972 adhered to shed urinary epithelial cells. Our results indicate that improving urinary catheter adherence augments the bacterial interference capabilities of benign E. coli 83972. Increased expression of type-1 fimbriae may enhance bacterial interference without conferring virulence on E. coli 83972.
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影响因子：
3.4
作者：
Bergsten, Goran;Wullt, Bjorn;Svanborg, Catharina
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2.1
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3.2
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2.2
作者：
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通讯作者：
Howard, L