Metagenomic Analysis of Fecal Archaea, Bacteria, Eukaryota, and Virus in Przewalski's Horses Following Anthelmintic Treatment.
Metagenomic Analysis of Fecal Archaea, Bacteria, Eukaryota, and Virus in Przewalski's Horses Following Anthelmintic Treatment.
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DOI：
10.3389/fvets.2021.708512
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发表时间：
2021
影响因子：
3.2
通讯作者：
Mok KM
中科院分区：
文献类型：
--
作者：
Hu D;Yang J;Qi Y;Li B;Li K;Mok KM
肠道微生物群参与宿主的免疫反应和新陈代谢。频繁使用驱虫化合物驱除寄生虫会扰乱马肠道微生物群。然而，大多数研究都是关于这种治疗对肠道细菌微生物的影响。没有一个涉及整个微生物群落，包括马科动物的古细菌、真核和病毒群落。本研究首次探讨普氏野马驱虫治疗前后微生物群落组成和结构的差异，并基于宏基因组测序确定其功能属性的相应变化。结果表明，古细菌中，产甲烷菌广古菌门是优势门。在该门下，驱虫治疗增加了甲烷短杆菌属，减少了甲烷小体属和另外两种主要古菌物种：实验室甲烷小体和巴瓦里甲烷小体。在细菌中，厚壁菌门和拟杆菌门是优势门。驱虫治疗增加了梭菌属和真杆菌属的数量，并减少了拟杆菌属和普雷沃氏菌属以及优势菌种的数量。这些改变的属与免疫和消化有关。在真核生物中，驱虫药治疗还改变了与消化相关的属，并大大降低了已识别物种的相对丰度。在病毒方面，驱虫药治疗增加了未分类_d__病毒属，并减少了未分类_f__管病毒科和未分类_f__肌病毒科的属。大多数已识别的病毒种类被归类为噬菌体，它们比其他病毒对驱虫治疗更敏感。此外，驱虫治疗被发现会增加与马的某些临床疾病相关的病原体数量。 COG和KEGG功能分析表明，普氏野马肠道菌群主要参与碳水化合物和氨基酸代谢。驱虫治疗并没有改变它们的整体功能；然而，它取代了参与每种功能或途径的功能微生物种群。这些结果提供了驱虫治疗对普氏野马肠道微生物群造成的变化的完整了解。
Intestinal microbiota is involved in immune response and metabolism of the host. The frequent use of anthelmintic compounds for parasite expulsion causes disturbance to the equine intestinal microbiota. However, most studies were on the effects of such treatment on the intestinal bacterial microbes; none is on the entire microbial community including archaea and eukaryotic and viral community in equine animals. This study is the first to explore the differences of the microbial community composition and structure in Przewalski's horses prior to and following anthelmintic treatment, and to determine the corresponding changes of their functional attributes based on metagenomic sequencing. Results showed that in archaea, the methanogen of Euryarchaeota was the dominant phylum. Under this phylum, anthelmintic treatment increased the Methanobrevibacter genus and decreased the Methanocorpusculum genus and two other dominant archaea species, Methanocorpusculum labreanum and Methanocorpusculum bavaricum. In bacteria, Firmicutes and Bacteroidetes were the dominant phyla. Anthelmintic treatment increased the genera of Clostridium and Eubacterium and decreased those of Bacteroides and Prevotella and dominant bacteria species. These altered genera were associated with immunity and digestion. In eukaryota, anthelmintic treatment also changed the genera related to digestion and substantially decreased the relative abundances of identified species. In virus, anthelmintic treatment increased the genus of unclassified_d__Viruses and decreased those of unclassified_f__Siphoviridae and unclassified_f__Myoviridae. Most of the identified viral species were classified into phage, which were more sensitive to anthelmintic treatment than other viruses. Furthermore, anthelmintic treatment was found to increase the number of pathogens related to some clinical diseases in horses. The COG and KEGG function analysis showed that the intestinal microbiota of Przewalski's horse mainly participated in the carbohydrate and amino acid metabolism. The anthelmintic treatment did not change their overall function; however, it displaced the population of the functional microbes involved in each function or pathway. These results provide a complete view on the changes caused by anthelmintic treatment in the intestinal microbiota of the Przewalski's horses.
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影响因子：
4.8
作者：
Carey RA;Montag D
通讯作者：
Montag D
DOI：
10.1093/bioinformatics/bts565
发表时间：
2012-12-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Fu L;Niu B;Zhu Z;Wu S;Li W
通讯作者：
Li W
影响因子：
15.5
作者：
Fricke WF;Song Y;Wang AJ;Smith A;Grinchuk V;Mongodin E;Pei C;Ma B;Lu N;Urban JF Jr;Shea-Donohue T;Zhao A
通讯作者：
Zhao A
影响因子：
4.3
作者：
Gaci, Nadia;Borrel, Guillaume;Brugere, Jean-Francois
通讯作者：
Brugere, Jean-Francois
影响因子：
--
作者：
Crotch-Harvey L;Thomas LA;Worgan HJ;Douglas JL;Gilby DE;McEwan NR
通讯作者：
McEwan NR