Larval diet affects mosquito development and permissiveness to Plasmodium infection.
Larval diet affects mosquito development and permissiveness to Plasmodium infection.
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DOI：
10.1038/srep38230
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发表时间：
2016-12-02
影响因子：
4.6
通讯作者：
Gendrin M
中科院分区：
文献类型：
--
作者：
Linenberg I;Christophides GK;Gendrin M
疟疾病媒蚊子的幼虫期在水池中发育，主要以微生物和环境碎屑为食。幼虫营养供给的丰富程度影响着幼虫和成虫的发育和代谢。本研究研究了不同饵料对柯氏按蚊发育、菌群含量及对疟原虫的容忍度的影响。我们在实验室里测试了三种经常用来饲养蚊子的鱼饲料，包括两种颗粒饲料，克拉克博士的池粒和西锦鱼丸，以及一种片状饲料，四合蛋白鱼片。与食用薄片相比，食用两种类型的颗粒的幼虫生长发育更快，产生更大的成虫。这与颗粒喂养的幼虫中较高的微生物群负荷有关，这与已知的微生物群对蚊子发育的积极作用一致。幼虫的饮食也显著影响成虫感染伯氏疟原虫的流行程度和强度，因此饲喂锦锦鱼丸的幼虫发育成对寄生虫高度耐受的成虫，在感染后存活时间更长。这与中肠菌群中肠杆菌科的数量较低有关。总之，我们的研究结果揭示了幼虫取食对蚊子发育、微生物群和媒介能力的影响；它们还为蚊子饲养提供了有用的数据。
The larval stages of malaria vector mosquitoes develop in water pools, feeding mostly on microorganisms and environmental detritus. Richness in the nutrient supply to larvae influences the development and metabolism of larvae and adults. Here, we investigated the effects of larval diet on the development, microbiota content and permissiveness to Plasmodium of Anopheles coluzzii. We tested three fish diets often used to rear mosquitoes in the laboratory, including two pelleted diets, Dr. Clarke’s Pool Pellets and Nishikoi Fish Pellets, and one flaked diet, Tetramin Fish-Flakes. Larvae grow and develop faster and produce bigger adults when feeding on both types of pellets compared with flakes. This correlates with a higher microbiota load in pellet-fed larvae, in agreement with the known positive effect of the microbiota on mosquito development. Larval diet also significantly influences the prevalence and intensity of Plasmodium berghei infection in adults, whereby Nishikoi Fish Pellets-fed larvae develop into adults that are highly permissive to parasites and survive longer after infection. This correlates with a lower amount of Enterobacteriaceae in the midgut microbiota. Together, our results shed light on the influence of larval feeding on mosquito development, microbiota and vector competence; they also provide useful data for mosquito rearing.
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影响因子：
4.2
作者：
Gendrin M;Yerbanga RS;Ouedraogo JB;Lefèvre T;Cohuet A;Christophides GK
通讯作者：
Christophides GK
影响因子：
4.6
作者：
Bando, Hironori;Okado, Kiyoshi;Guelbeogo, Wamdaogo M.;Badolo, Athanase;Aonuma, Hiroka;Nelson, Bryce;Fukumoto, Shinya;Xuan, Xuenan;Sagnon, N'Fale;Kanuka, Hirotaka
通讯作者：
Kanuka, Hirotaka
影响因子：
5.6
作者：
Douglas AE
通讯作者：
Douglas AE
影响因子：
3.2
作者：
Gimonneau, Geoffrey;Tchioffo, Majoline T.;Morlais, Isabelle
通讯作者：
Morlais, Isabelle
影响因子：
3.7
作者：
Couret J;Dotson E;Benedict MQ
通讯作者：
Benedict MQ