Neuronal activity and axonal sprouting differentially regulate CNTF and CNTF receptor complex in the rat supraoptic nucleus.
Neuronal activity and axonal sprouting differentially regulate CNTF and CNTF receptor complex in the rat supraoptic nucleus.
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DOI：
10.1016/j.expneurol.2011.10.009
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发表时间：
2012-01
影响因子：
5.3
通讯作者：
Watt, John A.
中科院分区：
文献类型：
--
作者：
Askvig, Jason M.;Leiphon, Laura J.;Watt, John A.
我们以前证明，下丘脑视上核（SON）经历了一个强大的轴突发芽反应后，下丘脑神经垂体束的单侧横切。伴随着这种反应的是睫状神经营养因子（CNTF）和CNTF受体α（CNTFRα）表达的增加，在对侧非未损伤的SON中轴突生长发生。虽然这些研究结果表明，CNTF可能作为一种生长因子，支持神经元可塑性的SON，它仍然有待确定，如果观察到的神经营养因子的表达增加与发芽反应本身或更普遍的增加神经分泌活动与损伤后的反应。因此，我们使用免疫细胞化学和蛋白质印迹分析来检查CNTF的表达和CNTF受体复合物的成分在发芽与刺激的SON。Western blot分析显示，在没有神经元损失的情况下，损伤后10天，新生SON中CNTF、CNTFRα和gp130蛋白水平显著增加，但LIFRβ蛋白水平没有增加。相反，在无损伤的情况下，神经分泌活性的渗透刺激导致CNTF蛋白水平显著降低，而CNTFRα、gp130或LIFRβ蛋白水平没有变化。免疫细胞化学分析进一步证实gp130定位于大细胞神经元和星形胶质细胞，而LIFRβ受体仅见于SON的星形胶质细胞。这些结果是一致的假设，增加CNTF和CNTFR复合物的发芽，代谢活性SON直接相关的发芽反应，而不是增加神经分泌活性。
We demonstrated previously that the hypothalamic supraoptic nucleus (SON) undergoes a robust axonal sprouting response following unilateral transection of the hypothalamo-neurohypophysial tract. Concomitant with this response is an increase in ciliary neurotrophic factor (CNTF) and CNTF receptor alpha (CNTFRα) expression in the contralateral non-uninjured SON from which the axonal outgrowth occurs. While these findings suggest that CNTF may act as a growth factor in support of neuronal plasticity in the SON, it remained to be determined if the observed increase in neurotrophin expression was related to the sprouting response per se or more generally to the increased neurosecretory activity associated with the post-lesion response. Therefore we used immunocytochemistry and Western blot analysis to examine the expression of CNTF and the components of the CNTF receptor complex in sprouting versus osmotically-stimulated SON. Western blot analysis revealed a significant increase in CNTF, CNTFRα, and gp130, but not LIFRβ, protein levels in the sprouting SON at 10 days post lesion in the absence of neuronal loss. In contrast, osmotic stimulation of neurosecretory activity in the absence of injury resulted in a significant decrease in CNTF protein levels with no change in CNTFRα, gp130, or LIFRβ protein levels. Immunocytochemical analysis further demonstrated gp130 localization on magnocellular neurons and astrocytes while the LIFRβ receptor was found only on astrocytes in the SON. These results are consistent with the hypothesis that increased CNTF and CNTFR complex in the sprouting, metabolically active SON are related directly to the sprouting response and not the increase in neurosecretory activity.
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影响因子：
5.3
作者：
LEIBOWITZ, SF;EIDELMAN, D;SLADEK, CD
通讯作者：
SLADEK, CD
影响因子：
3.3
作者：
DYBALL, REJ;KEMPLAY, SK
通讯作者：
KEMPLAY, SK
影响因子：
6.2
作者：
CARROLL, P;SENDTNER, M;THOENEN, H
通讯作者：
THOENEN, H
影响因子：
56.9
作者：
DAVIS, S;ALDRICH, TH;YANCOPOULOS, GD
通讯作者：
YANCOPOULOS, GD
影响因子：
3.3
作者：
Aliaga, E;Arancibia, S;Tapia-Arancibia, L
通讯作者：
Tapia-Arancibia, L