Mutations in Acetylcholinesterase2 (ace2) increase the insensitivity of acetylcholinesterase to fosthiazate in the root-knot nematode Meloidogyne incognita.
Mutations in Acetylcholinesterase2 (ace2) increase the insensitivity of acetylcholinesterase to fosthiazate in the root-knot nematode Meloidogyne incognita.
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乙酰胆碱酯酶 2 (ace2) 的突变增加了根结线虫根结线虫中乙酰胆碱酯酶对噻唑磷的不敏感性
DOI：
10.1038/srep38102
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发表时间：
2016-11-29
影响因子：
4.6
通讯作者：
Peng DL
中科院分区：
文献类型：
--
作者：
Huang WK;Wu QS;Peng H;Kong LA;Liu SM;Yin HQ;Cui RQ;Zhan LP;Cui JK;Peng DL
南方根结线虫（Meloidogyne incognita）对连作蔬菜危害严重。在我国，该线虫的防治主要依靠有机磷类杀线虫剂。在这里，我们描述了温室收集的M抗性种群（RP）和实验室易感种群（SP）对有机磷杀线虫剂噻嗪磷的抗性。无名氏实时荧光定量PCR结果显示，RP中乙酰胆碱酯酶2（acetylcholinesterase2，ace 2）的转录水平显著高于SP，在ace 2的cDNA片段中发现了18个非同义氨基酸的差异，RP中的乙酰胆碱酯酶（acetylcholinesterase，AChE）的转录水平显著高于SP。蛋白质活性在RP中显著低于在SP中。敲除ace2基因后，ace2转录水平显著降低，但未观察到对幼鱼感染的负面影响。RNA干扰RP种群的50%致死浓度降低了40%，但噻唑膦对RP种群AChE活性的抑制率显著提高。因此，在M. Incognita耐药人群与ACE 2突变密切相关。这些结果为深入了解根结线虫对有机磷类杀线虫剂的抗性机制提供了有价值的信息。
The root-knot nematode Meloidogyne incognita causes severe damage to continuously cropping vegetables. The control of this nematode relies heavily on organophosphate nematicides in China. Here, we described resistance to the organophosphate nematicide fosthiazate in a greenhouse-collected resistant population (RP) and a laboratory susceptible population (SP) of M. incognita. Fosthiazate was 2.74-fold less toxic to nematodes from RP than that from SP. Quantitative real-time PCR revealed that the acetylcholinesterase2 (ace2) transcription level in the RP was significantly higher than that in the SP. Eighteen nonsynonymous amino acid differences in ace2 were observed between the cDNA fragments of the RP and SP. The acetylcholinesterase (AChE) protein activity in the RP was significantly reduced compared with that in the SP. After knocking down the ace2 gene, the ace2 transcription level was significantly decreased, but no negative impact on the infection of juveniles was observed. The 50% lethal concentration of the RNAi RP population decreased 40%, but the inhibition rate of fosthiazate against AChE activity was significantly increased in RP population. Thus, the increased fosthiazate insensitivity in the M. incognita resistant population was strongly associated with mutations in ace2. These results provide valuable insights into the resistance mechanism of root-knot nematode to organophosphate nematicides.
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影响因子：
5.8
作者：
ELLMAN, GL;COURTNEY, KD;FEATHERSTONE, RM
通讯作者：
FEATHERSTONE, RM
DOI：
10.1016/j.bbrc.2006.12.088
发表时间：
2007-02-16
影响因子：
3.1
作者：
Lee, Dae-Weon;Choi, Jae Young;Koh, Young Ho
通讯作者：
Koh, Young Ho
影响因子：
5.1
作者：
Oakeshott, JG;Devonshire, AL;Russell, RJ
通讯作者：
Russell, RJ
影响因子：
4.2
作者：
Kiewnick, S.;Sikora, R. A.
通讯作者：
Sikora, R. A.
影响因子：
3.8
作者：
Kim, Young Ho;Lee, Si Hyeock
通讯作者：
Lee, Si Hyeock