Functional liposome loaded curcumin for the treatment of Streptococcus mutans biofilm.
Functional liposome loaded curcumin for the treatment of Streptococcus mutans biofilm.
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DOI：
10.3389/fchem.2023.1160521
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发表时间：
2023
影响因子：
5.5
通讯作者：
中科院分区：
文献类型：
--
作者：
牙菌斑生物膜在龋齿的发生发展中起着重要作用，主要由变形链球菌（链球菌）形成。抗生素治疗是控制菌斑的传统方法。然而，诸如药物渗透性差和抗生素耐药性等问题促使人们寻找替代策略。本文希望通过姜黄素（一种具有光动力效应的天然植物提取物）对变形链球菌的抗菌作用来避免抗生素耐药性。但姜黄素水溶性低，稳定性差，代谢率高，清除率快，生物利用度有限，限制了其临床应用。近年来，由于脂质体具有载药效率高、生物环境稳定性好、释放可控、生物相容性好、无毒、可生物降解等诸多优点，成为广泛应用的药物载体。因此，我们构建了姜黄素负载脂质体（Cur@LP）来避免姜黄素的缺陷。方法：Cur@LP与NHS作用后，通过缩合反应粘附在变形链球菌生物膜表面。通过透射电镜（TEM）和动态光散射（DLS）对脂质体（LP）和Cur@LP进行了表征。采用CCK-8法和LDH法评价Cur@LP的细胞毒性。用激光共聚焦扫描显微镜（CLSM）观察Cur@LP对变形链球菌生物膜的粘附情况。通过结晶紫染色、CLSM和扫描电镜（SEM）对Cur@LP的抗菌效果进行评价。结果：LP和Cur@LP的平均直径分别为206.67±8.38 nm和312±18.78 nm。LP和Cur@LP的ζ电位分别为~−19.3 mV和~−20.8 mV。Cur@LP包封率为（42.61±2.19）%，2 h时姜黄素快速释放量可达±21%，其细胞毒性可忽略，可有效粘附在变形链球菌生物膜上并抑制其生长。讨论：姜黄素在许多领域得到了广泛的研究，比如癌症，这可以归因于它的抗氧化和抗炎作用。目前，姜黄素在变形链球菌生物膜中的传递研究较少。在本研究中，我们验证了Cur@LP对变形链球菌生物膜的粘附性和抗菌性。这种生物膜去除策略有可能被转化为临床。
Introduction: Plaque biofilms, mainly formed by Streptococcus mutans (S. mutans), play an important role in the occurrence and development of dental caries. Antibiotic treatment is the traditional way to control plaque. However, problems such as poor drug penetration and antibiotic resistance have encouraged the search for alternative strategies. In this paper, we hope to avoid antibiotic resistance through the antibacterial effect of curcumin, a natural plant extract with photodynamic effects, on S. mutans. However, the clinical application of curcumin is limited due to its low water solubility, poor stability, high metabolic rate, fast clearance rate, and limited bioavailability. In recent years, liposomes have become a widely used drug carrier due to their numerous advantages, such as high drug loading efficiency, high stability in the biological environment, controlled release, biocompatibility, non-toxic, and biodegradability. So, we constructed a curcumin-loaded liposome (Cur@LP) to avoid the defect of curcumin. Methods: Cur@LP functioned with NHS can adhere to the surface of the S. mutans biofilm by condensation reaction. Liposome (LP) and Cur@LP was characterized by transmission electron microscopy (TEM) and dynamic light scattering (DLS). The cytotoxicity of Cur@LP was evaluated by CCK-8 assay and LDH assay. The adhesion of Cur@LP to S. mutans biofilm was observed by confocal laser scanning microscope (CLSM). The antibiofilm efficiency of Cur@LP were evaluated by crystal violet staining, CLSM, and scanning electron microscope (SEM). Results: The mean diameter of LP and Cur@LP were 206.67 ± 8.38 nm and 312 ± 18.78 nm respectively. The ζ-potential of LP and Cur@LP were ∼−19.3 mV and ∼−20.8 mV respectively. The encapsulation efficiency of Cur@LP was (42.61 ± 2.19) %, and curcumin was rapidly released up to ±21% at 2 h. Cur@LP has negligible cytotoxicity, and can effectively adhered to the S. mutans biofilm and inhibited its growth. Discussion: Curcumin has been widely studied in many fields such as cancer, which can be attributed to its antioxidant and anti-inflammatory effects. At present, there are few studies on the delivery of curcumin to S. mutans biofilm. In this study, we verified the adhesion and antibiofilm of Cur@LP to S. mutans biofilm. This biofilm removal strategy has the potential to be translated into the clinic.
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影响因子：
5.9
作者：
Forssten SD;Björklund M;Ouwehand AC
通讯作者：
Ouwehand AC
影响因子：
6.1
作者：
Maheshwari, RK;Singh, AK;Srimal, RC
通讯作者：
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在小鼠体内和人类离体感染模型中评估了具有缀合抗菌剂的纳米载体可消除致病性生物膜
影响因子：
9.7
作者：
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通讯作者：
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影响因子：
2.9
作者：
Agarwal, Nidhi Bharal;Jain, Seema;Sharma, Krishna K.
通讯作者：
Sharma, Krishna K.
影响因子：
2.6
作者：
Pemberton, M. N.;Gibson, J.
通讯作者：
Gibson, J.