Analysis of ESTs from Lutzomyia longipalpis sand flies and their contribution toward understanding the insect-parasite relationship.
Analysis of ESTs from Lutzomyia longipalpis sand flies and their contribution toward understanding the insect-parasite relationship.
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DOI：
10.1016/j.ygeno.2006.06.011
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发表时间：
2006-12
期刊：
影响因子：
4.4
通讯作者：
Bates, Paul A.
中科院分区：
文献类型：
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作者：
Dillon, Rod J.;Ivens, Al C.;Churcher, Carol;Holroyd, Nancy;Quail, Michael A.;Rogers, Matthew E.;Soares, M. Bento;Bonaldo, Maria F.;Casavant, Thomas L.;Lehane, Mike J.;Bates, Paul A.
表达序列标签文库已经从内脏利什曼病的白蛉载体长须路蝇（Lutzomyia longipalpis）产生。从整个成虫构建标准化cDNA文库，从两端对16，608个克隆进行测序，并组装成10，203个重叠群和单联体。其中58%与其他生物的已知基因有显著的相似性，< 4%与描述的白蛉基因相同，42%与任何数据库序列都不匹配。我们的分析揭示了参与肠道屏障功能（围肌肽、微绒毛蛋白、谷氨酰胺合酶）、消化生理学（分泌和膜锚定水解酶）和免疫反应（革兰氏阴性结合蛋白、硫酯蛋白、清道夫受体、半乳糖凝集素、信号传导途径因子、半胱天冬酶、丝氨酸蛋白酶抑制剂和过氧化物酶）的推定蛋白质。该转录组数据集的序列分析为可能与载体对利什曼原虫发展的反应相关的基因提供了新的见解。
An expressed sequence tag library has been generated from a sand fly vector of visceral leishmaniasis, Lutzomyia longipalpis. A normalized cDNA library was constructed from whole adults and 16,608 clones were sequenced from both ends and assembled into 10,203 contigs and singlets. Of these 58% showed significant similarity to known genes from other organisms, < 4% were identical to described sand fly genes, and 42% had no match to any database sequence. Our analyses revealed putative proteins involved in the barrier function of the gut (peritrophins, microvillar proteins, glutamine synthase), digestive physiology (secreted and membrane-anchored hydrolytic enzymes), and the immune response (gram-negative binding proteins, thioester proteins, scavenger receptors, galectins, signaling pathway factors, caspases, serpins, and peroxidases). Sequence analysis of this transcriptome dataset has provided new insights into genes that might be associated with the response of the vector to the development of Leishmania.
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影响因子：
56.9
作者：
Ivens, AC;Peacock, CS;Myler, PJ
通讯作者：
Myler, PJ
影响因子：
3.8
作者：
Hao, ZG;Kasumba, I;Aksoy, S
通讯作者：
Aksoy, S
影响因子：
2.9
作者：
Choi, JY;Whitten, MMA;Lee, BL
通讯作者：
Lee, BL
影响因子：
56.9
作者：
Christophides, GK;Zdobnov, E;Kafatos, FC
通讯作者：
Kafatos, FC
DOI：
10.1046/j.1432-1033.2003.03812.x
发表时间：
2003-11-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Bauer, H;Gromer, S;Müller, HM
通讯作者：
Müller, HM