Biodegradable electrospun nanofibers coated with platelet-rich plasma for cell adhesion and proliferation.
Biodegradable electrospun nanofibers coated with platelet-rich plasma for cell adhesion and proliferation.
复制标题
DOI：
10.1016/j.msec.2014.03.065
复制
发表时间：
2014-07-01
影响因子：
7.9
通讯作者：
Macossay, Javier
中科院分区：
文献类型：
--
作者：
Diaz-Gomez, Luis;Alvarez-Lorenzo, Carmen;Concheiro, Angel;Silva, Maite;Dominguez, Fernando;Sheikh, Faheem A.;Cantu, Travis;Desai, Raj;Garcia, Vanessa L.;Macossay, Javier
关键词：
采用富血小板血浆（PRP）对电纺聚己内酯（PCL）生物可降解支架进行表面处理，以提高细胞的粘附和增殖能力。从人血沉棕黄层获得PRP，并在人脂肪来源的间充质干细胞（MSC）上进行测试，以确认细胞增殖和细胞相容性。然后，PRP通过冻干吸附在PCL支架上，这导致2.85（s.d.）0.14）mg/mg。评价支架的力学性能（杨氏模量、拉伸应力和拉伸应变）、总蛋白和生长因子（PDGF-BB、TGF-β1和VEGF）的持续释放以及血液相容性。与原始PCL纤维相比，接种在PRP-PCL纳米纤维上的MSC显示出增加的粘附和增殖。此外，吸附的PRP使血管生成的功能，在鸡绒毛尿囊膜（CAM）模型中观察到的新血管形成。总的来说，这些结果表明PRP-PCL支架有望用于组织再生应用。
Biodegradable electrospun poly(ε-caprolactone) (PCL) scaffolds were coated with platelet-rich plasma (PRP) to improve cell adhesion and proliferation. PRP was obtained from human buffy coat, and tested on human adipose-derived mesenchymal stem cells (MSC) to confirm cell proliferation and cytocompatibility. Then, PRP was adsorbed on the PCL scaffolds via lyophilization, which resulted in uniform sponge-like coating of 2.85 (s.d. 0.14) mg/mg. The scaffolds were evaluated regarding mechanical properties (Young’s modulus, tensile stress and tensile strain), sustained release of total protein and growth factors (PDGF-BB, TGF-β1 and VEGF), and hemocompatibility. MSC seeded on the PRP-PCL nanofibers showed an increased adhesion and proliferation compared to pristine PCL fibers. Moreover, the adsorbed PRP enabled angiogenesis features observed as neovascularization in a chicken chorioallantoic membrane (CAM) model. Overall, these results suggest that PRP-PCL scaffolds hold promise for tissue regeneration applications.
登录
查看更多内容
影响因子：
14
作者：
Lee J;Yoo JJ;Atala A;Lee SJ
通讯作者：
Lee SJ
影响因子：
14
作者：
Luong-Van, E;Grondahl, L;Cool, SM
通讯作者：
Cool, SM
DOI：
10.1590/s1984-82502009000400021
发表时间：
2009-12-01
影响因子：
1.3
作者：
Cazedey, Edith Cristina Laignier;Carvalho, Flávia Chiva;Salgado, Hérida Regina Nunes
通讯作者：
Salgado, Hérida Regina Nunes
DOI：
10.1002/jbm.b.32806
发表时间：
2013-01-01
影响因子：
3.4
作者：
Kutlu, Burak;Aydin, Rahime Seda Tigli;Nohutcu, Rahime M.
通讯作者：
Nohutcu, Rahime M.
影响因子：
1.8
作者：
Kilian, O;Alt, V;Schnettler, R
通讯作者：
Schnettler, R