Rotator cuff repair using a bioresorbable nanofiber interposition scaffold: a biomechanical and histologic analysis in sheep.
Rotator cuff repair using a bioresorbable nanofiber interposition scaffold: a biomechanical and histologic analysis in sheep.
复制标题
DOI：
10.1016/j.jse.2021.07.018
复制
发表时间：
2022-03
影响因子：
3
通讯作者：
McGilvray, Kirk
中科院分区：
文献类型：
--
作者：
Romeo, Anthony;Easley, Jeremiah;Regan, Dan;Hackett, Eileen;Johnson, James;Johnson, Jed;Puttlitz, Christian;McGilvray, Kirk
本研究的目的是在急性绵羊模型中评估由聚乙醇酸（PGA）和聚-L-丙交酯-共-ε-己内酯（PLCL）组成的插入式电纺纳米纤维支架增强的肩袖修复的机械、结构和组织学质量。使用双排经骨等效锚定技术在绵羊冈下肌模型中进行了 40 次急性冈下肌腱脱离和修复手术，该技术使用由聚乙醇酸-聚-L-丙交酯-共-ε-己内酯组成的插入纳米纤维支架或不使用支架。在术后 6 周（20 个样本）和 12 周（20 个样本）时间点对动物实施安乐死，以评估修复的生物力学和组织学特性并比较每组内的差异。在支架处理组中，最终失效力（以牛顿为单位）从 6 周到 12 周显着增加（P < .01），最终失效载荷从 6 周到 12 周显着增加（P < .01），最终失效应力（以兆帕为单位）从 6 周到 12 周显着增加（P < .01）。 6 周时，肌腱-骨附着与“间接”插入类型最为一致，而 12 周时，观察到附着点的进展和重建存在明显差异。与对照组相比，支架处理组的动物表现出纤维肌腱的插入与肱骨足迹开始以类似于绵羊冈下肌腱的“天然”直接/纤维软骨插入的方式组织。在大多数用支架治疗的动物中，存在与夏皮纤维类似的突出的穿孔胶原纤维，并延伸穿过钙化纤维软骨区域并附着在肱骨足迹上。 40 只羊均未出现手术并发症，包括伤口愈合延迟或感染。在绵羊急性肩袖修复模型中，使用双排经骨等效锚固固定技术将纳米纤维支架固定在肌腱和骨骼之间，可产生更大的失效强度。此外，在附着处观察到夏皮纤维样附着物（即从肌腱延伸到肱骨钙化纤维软骨中的胶原纤维），这在对照组中未见。基础科学研究；生物力学/组织学
The purpose of this study was to evaluate the mechanical, structural, and histologic quality of rotator cuff repairs augmented with an interposition electrospun nanofiber scaffold composed of polyglycolic acid (PGA) and poly-L-lactide-co-ε-caprolactone (PLCL) in an acute sheep model. Forty acute infraspinatus tendon detachment and repair procedures were performed in a sheep infraspinatus model using a double-row transosseous-equivalent anchor technique either with an interposition nanofiber scaffold composed of polyglycolic acid–poly-L-lactide-co-ε-caprolactone or with no scaffold. Animals were euthanized at the 6-week (20 samples) and 12-week (20 samples) postoperative time points to assess the biomechanical and histologic properties of the repairs and to compare differences within each group. Within the scaffold-treated group, there was a significant increase in ultimate failure force (in newtons) from 6 to 12 weeks (P < .01), a significant increase in ultimate failure load from 6 to 12 weeks (P < .01), and a significant increase in ultimate failure stress (in megapascals) from 6 to 12 weeks (P < .01). At 6 weeks, the tendon-bone attachment was most consistent with an “indirect” type of insertion, whereas at 12 weeks, a visible difference in the progression and re-formation of the enthesis was observed. Compared with controls, animals in the scaffold-treated group displayed an insertion of the fibrous tendon with the humeral footprint that was beginning to be organized in a manner similar to the “native” direct/fibrocartilaginous insertion of the ovine infraspinatus tendon. In the majority of these animals treated with the scaffold, prominent perforating collagen fibers, similar to Sharpey fibers, were present and extending through a region of calcified fibrocartilage and attaching to the humeral footprint. No surgical complications occurred in any of the 40 sheep, including delayed wound healing or infection. In a sheep acute rotator cuff repair model, securing a nanofiber scaffold between the tendon and the bone using a double-row transosseous-equivalent anchor fixation technique resulted in greater failure strength. Additionally, at the enthesis, Sharpey fiber–like attachments (ie, collagen fibers extending from the tendon into the calcified fibrocartilage of the humerus) were observed, which were not seen in the control group. Basic Science Study; Biomechanical/Histology
登录
查看更多内容
用于血管组织工程的电纺明胶/PCL 和胶原/PLCL 支架
影响因子：
8
作者：
Fu W;Liu Z;Feng B;Hu R;He X;Wang H;Yin M;Huang H;Zhang H;Wang W
通讯作者：
Wang W
影响因子：
4.6
作者：
Carr, A.;Cooper, C.;Ramsay, C.
通讯作者：
Ramsay, C.
影响因子：
--
作者：
Luan, Tammy;Liu, Xuhui;Feeley, Brian T.
通讯作者：
Feeley, Brian T.
影响因子：
3.8
作者：
Lampe, Kyle J.;Namba, Rachael M.;Silverman, Tyler R.;Bjugstad, Kimberly B.;Mahoney, Melissa J.
通讯作者：
Mahoney, Melissa J.
影响因子：
3
作者：
Easley, Jeremiah;Puttlitz, Christian;McGilvray, Kirk
通讯作者：
McGilvray, Kirk