Impact of lactic acid on cell proliferation and free radical-induced cell death in monolayer cultures of neural precursor cells.
Impact of lactic acid on cell proliferation and free radical-induced cell death in monolayer cultures of neural precursor cells.
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DOI：
10.1002/bit.22352
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发表时间：
2009-08-15
影响因子：
3.8
通讯作者：
Mahoney, Melissa J.
中科院分区：
文献类型：
--
作者：
Lampe, Kyle J.;Namba, Rachael M.;Silverman, Tyler R.;Bjugstad, Kimberly B.;Mahoney, Melissa J.
由乳酸和乙醇酸的聚酯或两者的混合物制备的生物材料在存在水的情况下降解为天然存在的代谢物乳酸和乙醇酸。虽然从生物材料释放的乳酸降解产物被身体良好耐受，但乳酸可以影响细胞的代谢功能;它可以作为细胞的能量底物，并且已被证明具有抗氧化特性。神经前体细胞，作为神经组织再生策略的细胞来源的相当感兴趣的细胞群体，产生大量的活性氧，并且当与可降解生物材料结合时，可能受到释放的乳酸的影响。在这项工作中，乳酸对神经细胞群含有增殖神经前体细胞的影响进行了检查，在单层培养。乳酸被发现是在过氧化氢或光引发剂（I2959）的存在下产生的非外源性自由基，通常用于制备光聚合生物材料。除了其对外源性添加的自由基的影响外，乳酸还降低了细胞内的氧化还原状态，增加了细胞群的增殖，并改变了细胞组成。这项研究的结果提供了对乳酸在神经细胞发育中自然发挥的作用的深入了解，也引起了生物材料科学家的兴趣，这些科学家专注于开发用于细胞培养装置的可降解乳酸基聚合物。乳酸对其他细胞群的影响可能不同，应进行表征，以最好地了解细胞在可降解细胞培养装置中的功能。
Biomaterials prepared from polyesters of lactic acid and glycolic acid, or a mixture of the two, degrade in the presence of water into the naturally occurring metabolites, lactic acid and glycolic acid. While the lactic acid degradation product that is released from biomaterials is well-tolerated by the body, lactic acid can influence the metabolic function of cells; it can serve as an energy substrate for cells, and has been shown to have antioxidant properties. Neural precursor cells, a cell population of considerable interest as a source of cells for neural tissue regeneration strategies, generate a high amount of reactive oxygen species, and when associated with a degradable biomaterial, may be impacted by released lactic acid. In this work, the effect of lactic acid on a neural cell population containing proliferative neural precursor cells was examined in monolayer culture. Lactic acid was found to scavenge exogenously added free radicals produced in the presence of either hydrogen peroxide or a photoinitiator (I2959) commonly utilized in the preparation of photopolymerizable biomaterials. In addition to its effect on exogenously added free radicals, lactic acid reduced intracellular redox state, increased the proliferation of the cell population, and modified the cell composition. The findings of this study provide insight into the role that lactic acid plays naturally on developing neural cells and are also of interest to biomaterials scientists that are focused on the development of degradable lactic-acid based polymers for cell culture devices. The effect of lactic acid on other cell populations may differ and should be characterized to best understand how cells function in degradable cell culture devices.
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DOI：
10.1073/pnas.170209797
发表时间：
2000-08-29
影响因子：
11.1
作者：
Smith, J;Ladi, E;Noble, M
通讯作者：
Noble, M
影响因子：
3.3
作者：
Herz, H;Blake, DR;Grootveld, M
通讯作者：
Grootveld, M
影响因子：
3.5
作者：
Tsatmali, Marina;Walcott, Elisabeth C.;Crossin, Kathryn L.
通讯作者：
Crossin, Kathryn L.
影响因子：
56.9
作者：
LINDVALL, O;BRUNDIN, P;BJORKLUND, A
通讯作者：
BJORKLUND, A
影响因子：
56.9
作者：
FRIDOVICH, I
通讯作者：
FRIDOVICH, I