Phylogenetically diverse endozoic fungi in the South China Sea sponges and their potential in synthesizing bioactive natural products suggested by PKS gene and cytotoxic activity analysis
Phylogenetically diverse endozoic fungi in the South China Sea sponges and their potential in synthesizing bioactive natural products suggested by PKS gene and cytotoxic activity analysis
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PKS基因和细胞毒活性分析表明南海海绵内生真菌的系统发育多样性及其合成生物活性天然产物的潜力
DOI：
10.1007/s13225-012-0192-7
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发表时间：
2012
期刊：
影响因子：
20.3
通讯作者：
孙伟
中科院分区：
文献类型：
--
作者：
孙伟
有充分记录表明海绵含有大量微生物。尽管众所周知海绵衍生真菌是海洋天然产物的重要来源，但海绵相关真菌的系统发育多样性和生物学功能仍然很大程度上未知。本研究基于ITS系统发育分析揭示了南海海绵中可培养内生真菌的多样性。同时通过聚酮合酶（PKS）基因簇中β-酮合酶的检测和细胞毒活性生物测定来评估真菌生产生物活性天然产物的潜力。结果，包括10目14属（曲霉属、青霉属、头霉属、散霉属、链格孢属、镰刀菌属、肉座菌目、耶氏酵母属、念珠菌属、Hypoxylon、Sporidiobolus、Schizophyllum、Bjerkandera和Trichosporon）等多种真菌子囊菌门和担子菌门(Xylariales、Moniliales、Pleosporales、Saccharomycetales、Hypocreales、Eurotiales、Sporidiobolales、伞菌目、Aphyllophorales 和银耳目)被分离出来，其中曲霉属作为可培养真菌群落中的主要成分。裂褶菌属、孢子菌属、Bjerkanderin 门担子菌门和Yarrowia 门子囊菌门属首次从海绵中分离得到。在 12 个分离株中检测到 PKS 基因，表明它们具有合成 PKS 化合物的潜力。在12个带有PKS基因的分离株中，9个分离株对人癌细胞系A-549、Bel-7402、A-375和MRC-5表现出较强的体外细胞毒活性（例如IC50< 50μg/ml）。这项研究展示了南海海绵中具有系统发育多样性的内生真菌，并强调了海绵相关真菌在生产具有生物活性的天然产物方面的潜力。
Sponges are well documented to harbor large amounts of microbes. Though it is known that sponge-derived fungi are important sources for marine natural products, the phylogenetic diversity and biological function of sponge-associated fungi remain largely unknown. In this study, the diversity of culturable endozoic fungi in sponges from the South China Sea was revealed based on the ITS phylogenetic analysis. Meanwhile the fungal potential for producing bioactive natural products was estimated according to the detection of Beta-ketosynthase in the polyketide synthase (PKS) gene cluster and cytotoxic activity bioassay. As a result, diverse fungi including 14 genera (Aspergillus,Penicillium,Scolecobasidium, Eurotium,Alternaria,Fusarium, Hypocreales, Yarrowia,Candida, Hypoxylon,Sporidiobolus,Schizophyllum,Bjerkandera,andTrichosporon) in ten orders (Xylariales,Moniliales,Pleosporales,Saccharomycetales,Hypocreales,Eurotiales,Sporidiobolales,Agaricales,Aphyllophorales and Tremellales) of phylaAscomycotaandBasidiomycotawere isolated withAspergillusas the predominant component in the culturable fungal community.Particularly, generaSchizophyllum,Sporidiobolus, andBjerkanderain phylumBasidiomycotaand genusYarrowiain phylumAscomycotawere isolated from marine sponges for the first time. PKS genes were detected in 12 isolates suggesting their potential for synthesizing PKS compounds. Among the 12 isolates with PKS genes, 9 isolates displayed strong in vitro cytotoxic activity (e.g. IC50< 50 μg/ml) against human cancer cell lines A-549, Bel-7402, A-375 and MRC-5. This study demonstrates the phylogenetically diverse endozoic fungi in South China Sea sponges, and highlights the potential of sponge-associated fungi in producing biologically active natural products.
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影响因子：
3.4
作者：
Rot, Chagai;Goldfarb, Itay;Ilan, Micha;Huchon, Dorothee
通讯作者：
Huchon, Dorothee
从海洋海绵Tethya Aurantium分离的真菌的系统发育鉴定以及其继发代谢产物的鉴定。
影响因子：
5.4
作者：
Wiese J;Ohlendorf B;Blümel M;Schmaljohann R;Imhoff JF
通讯作者：
Imhoff JF
影响因子：
14.9
作者：
Pruesse E;Quast C;Knittel K;Fuchs BM;Ludwig W;Peplies J;Glöckner FO
通讯作者：
Glöckner FO
影响因子：
5.1
作者：
Brauers, G;Edrada, RA;Schaumann, K
通讯作者：
Schaumann, K
影响因子：
1.8
作者：
Abrell, LM;Borgeson, B;Crews, P
通讯作者：
Crews, P