An inventory of mucin genes in the chicken genome shows that the mucin domain of Muc13 is encoded by multiple exons and that ovomucin is part of a locus of related gel-forming mucins.
An inventory of mucin genes in the chicken genome shows that the mucin domain of Muc13 is encoded by multiple exons and that ovomucin is part of a locus of related gel-forming mucins.
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DOI：
10.1186/1471-2164-7-197
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发表时间：
2006-08-03
期刊：
影响因子：
4.4
通讯作者：
Samuelsson T
中科院分区：
文献类型：
--
作者：
Lang T;Hansson GC;Samuelsson T
粘蛋白是覆盖在身体上皮表面的大糖蛋白。所有粘蛋白都含有至少一个PTS结构域，这是一个富含脯氨酸、苏氨酸和丝氨酸的区域。粘蛋白还具有冯氏血友病（VWD）结构域或SEA结构域。我们已经开发了基于这些蛋白质特性的计算方法来识别粘蛋白基因和蛋白质。使用这种方法，我们能够表征不同的生物体，其中基因组序列是相对于他们的粘蛋白库可用的。我们在此对鸡（Gallus Gallus）基因组编码的潜在粘蛋白进行了全面分析。鉴定出3种跨膜粘蛋白（Muc4、Muc13和Muc16）和4种凝胶形成粘蛋白（Muc6、Muc2、Muc5ac和Muc5b）。凝胶形成粘蛋白的编码位点类似于相应的人类粘蛋白。然而，鸡在Muc2和Muc5ac之间插入了一个额外的基因，该基因编码卵泡蛋白的α-亚基，这是一种类似于Muc2的蛋白质，但缺乏PTS结构域。我们还发现卵泡蛋白的β-亚基与人MUC6同源。跨膜Muc13基因在鸡和哺乳动物中与HEG（玻璃心）基因相邻。HEG具有PTS、EGF和Muc13等跨膜结构域，表明这两种蛋白具有进化相关性。与先前已知的粘蛋白不同，Muc13的PTS结构域由多个外显子编码，其中每个外显子编码PTS结构域的一个重复单元。我们在鸡中报道了新的粘蛋白同源物，这一信息将有助于理解粘蛋白在脊椎动物中的进化。卵泡蛋白，一种在哺乳动物中没有发现的蛋白质，与其他凝胶形成粘蛋白位于同一位点，这一事实有力地支持了这些蛋白质与进化有关。此外，根据HEG和Muc13的生化特性以及它们在同一位点的存在，提出了HEG和Muc13之间的关系。最后，我们发现鸡Muc13分布在多个外显子之间，这提出了一种有趣的可能性，即PTS结构域的长度可以通过选择性剪接来控制。
Mucins are large glycoproteins that cover epithelial surfaces of the body. All mucins contain at least one PTS domain, a region rich in proline, threonine and serine. Mucins are also characterized by von Willebrand D (VWD) domains or SEA domains. We have developed computational methods to identify mucin genes and proteins based on these properties of the proteins. Using such methods we are able to characterize different organisms where genome sequence is available with respect to their mucin repertoire. We have here made a comprehensive analysis of potential mucins encoded by the chicken (Gallus gallus) genome. Three transmembrane mucins (Muc4, Muc13, and Muc16) and four gel-forming mucins (Muc6, Muc2, Muc5ac, and Muc5b) were identified. The gel-forming mucins are encoded within a locus similar to the corresponding human mucins. However, the chicken has an additional gene inserted between Muc2 and Muc5ac that encodes the the α-subunit of ovomucin, a protein similar to Muc2, but it is lacking a PTS domain. We also show that the β-subunit of ovomucin is the orthologue of human MUC6. The transmembrane Muc13 gene is in chicken as well as in mammals adjacent to the HEG (heart of glass) gene. HEG has PTS, EGF and transmembrane domains like Muc13, suggesting that these two proteins are evolutionary related. Unlike previously known mucins, the PTS domain of Muc13 is encoded by multiple exons, where each exon encodes a repeat unit of the PTS domain. We report new mucin homologues in chicken and this information will aid in understanding the evolution of mucins in vertebrates. The fact that ovomucin, a protein not found in mammals, was located in the same locus as other gel-forming mucins provides strong support that these proteins are evolutionary related. Furthermore, a relationship of HEG and the transmembrane Muc13 is suggested on the basis of their biochemical properties and their presence in the same locus. Finally, our finding that the chicken Muc13 is distributed between multiple exons raises the interesting possibility that the length of the PTS domain could be controlled by alternative splicing.
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影响因子：
4.4
作者：
Lang T;Hansson GC;Samuelsson T
通讯作者：
Samuelsson T
影响因子：
3.4
作者：
Khatri, IA;Forstner, GG;Forstner, JF
通讯作者：
Forstner, JF
影响因子：
4.8
作者：
Axelsson, MAB;Asker, N;Hansson, GC
通讯作者：
Hansson, GC
DOI：
10.1006/bbrc.2002.6475
发表时间：
2002-03-01
影响因子：
3.1
作者：
Gum, JR;Crawley, SC;Kim, YS
通讯作者：
Kim, YS
DOI：
10.1006/bbrc.2000.3406
发表时间：
2000-09-07
影响因子：
3.1
作者：
Pratt, WS;Crawley, S;Swallow, DM
通讯作者：
Swallow, DM