Sequence variation in Toxoplasma gondii MIC13 gene among isolates from different hosts and geographical locations
Sequence variation in Toxoplasma gondii MIC13 gene among isolates from different hosts and geographical locations
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不同宿主和地理位置的弓形虫MIC13基因序列变异
DOI：
10.5897/ajmr12.040
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发表时间：
2012-02
影响因子：
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通讯作者：
Feng-Cai Zou
中科院分区：
文献类型：
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作者：
Ren Di;Zi-Guo Yuan;Min-Jun Xu;Yang Zhou;Jian-Fa Yang;Ze-Ping Lin;Rui-Qing Lin;Song-Ming Wu;Shao-Qi Lin;Feng-Cai Zou
弓形虫是尖端复合门的一种真核寄生虫，几乎可以感染任何有核哺乳动物和禽类细胞。微线虫是顶端复合体寄生虫的亚细胞器，可以分泌微线虫蛋白，在弓形虫的入侵过程中起着关键作用，是潜在的弓形虫病疫苗候选分子。在这项工作中，我们通过聚合酶链式反应扩增和随后的序列分析，检测了来自不同宿主和地理位置的18株弓形虫微丝蛋白13(TgMIC13)基因序列的变异。TgMIC13全长DNA序列约为2506~2507个碱基，cDNA序列约为1407个碱基，在不同的弓形虫分离株中高度保守，基因组DNA的种内变异仅为0.84%(21/2507)，而cDNA序列的种内变异高达0.71%(10/1407)。最大简约度(MP)系统发育分析表明，TgMIC13 DNA序列不能为不同宿主和不同地理位置的弓形虫分离株的种内系统发育分析和鉴别提供有效的分子标记。然而，结果表明，TgMIC13基因在不同弓形虫分离株之间的序列差异很小，这可能是进一步研究弓形虫病疫苗候选分子的一个有用的特征。
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关键词：弓形虫、弓形虫病、微体蛋白13(MIC13)、序列变异、系统发育分析。
Toxoplasma gondii, an eukaryotic parasite of the phylum Apicomplexa, can infect almost any nucleated mammalian and avian cells. Micronemes are sub-cellular organelles of apicomplexan parasites which can secrete microneme proteins (MICs) playing a key role in the invasion process of T. gondii and are potential vaccine candidate molecules against toxoplasmosis. In this work, we examined sequence variation in T. gondii microneme protein 13 (TgMIC13) gene sequences among 18 isolates from different hosts and geographical locations by PCR amplification and subsequent sequence analysis. The complete TgMIC13 DNA and cDNA sequences were 2506-2507 and 1407 bp, respectively, and they were quite conserved among the T. gondii isolates, with intra-specific variation of only up to 0.84% (21/2507) for genomic DNA and up to 0.71% (10/1407) for cDNA sequences. Phylogenetic analysis using maximum parsimony (MP) revealed that the TgMIC13 DNA sequences were not able to provide an effective molecular marker for intra-species phylogenetic analysis and differential identification of T. gondii isolates from different hosts and geographical locations. However, the results demonstrated that sequence variation in TgMIC13 gene was quite low among different T. gondii isolates, which may be a useful feature as an anti-toxoplasmosis vaccine candidate molecule in further studies.
Key words: Toxoplasma gondii, toxoplasmosis, microneme protein 13 (MIC13), sequence variation, phylogenetic analysis.
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影响因子：
4
作者：
Weiss LM;Dubey JP
通讯作者：
Dubey JP
影响因子：
1.9
作者：
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通讯作者：
Zhu, Xing-Quan
影响因子：
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作者：
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影响因子：
6.6
作者：
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通讯作者：
V. Carruthers;L. Sibley
影响因子：
3.2
作者：
Zhou P;Chen Z;Li HL;Zheng H;He S;Lin RQ;Zhu XQ
通讯作者：
Zhu XQ