Comprehensive analysis of differently expressed genes and proteins in albino and green plantlets from a wheat anther culture
Comprehensive analysis of differently expressed genes and proteins in albino and green plantlets from a wheat anther culture
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小麦花药培养物白化和绿色植株差异表达基因和蛋白质的综合分析
DOI：
10.1007/s10535-016-0662-y
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发表时间：
2017-06
影响因子：
1.5
通讯作者：
Ye X. G.
中科院分区：
文献类型：
--
作者：
Zhao P.;Wang K.;Zhang W.;Liu H. Y.;Du L. P.;Hu H. R.;Ye X. G.
花药培养技术虽然已广泛应用于小麦育种项目中，但其白化病率较高，限制了其应用。白化病的机制尚未在分子水平上得到明确研究。在这项研究中，使用转录组测序（RNA-Seq）、二维电泳和质谱分析和比较了小麦花药培养物中白化幼苗和绿色幼苗的差异表达基因（DEG）和蛋白质（DEP）。以白化组文库为对照，共鉴定出1 892个DEG（1 115个下调，777个上调）和43个DEP（15个下调，28个上调）主要参与光合途径以及卟啉和叶绿素代谢。通过基因本体分析确定，大多数 DEG 参与了类囊体和叶绿体包膜的构建。使用实时定量PCR评估12个选定基因的表达模式，结果与RNA-Seq分析一致。我们的结果可能有助于开发新方法来降低小麦花药培养中的白化幼苗率。
The anther culture technique, although it has been widely used in wheat breeding programs, is associated with a high albinism rate that limits its application. The mechanism of albinism has not been studied explicitly at the molecular level. In this study, differently expressed genes (DEGs) and proteins (DEPs) of albino and green seedlings from a wheat anther culture were analyzed and compared using transcriptomic sequencing (RNA-Seq), two-dimensional electrophoresis, and mass spectrometry. A total of 1 892 DEGs (1 115 down-regulated and 777 up-regulated) and 43 DEPs (15 down-regulated and 28 up-regulated) that are primarily involved in photosynthetic pathways and porphyrin and chlorophyll metabolism was identified when the albino group library was used as the control. Most DEGs participated in constructing components of thylakoids and the chloroplast envelope as determinedviagene ontology analysis. The expression patterns of 12 selected genes were evaluated using real-time quantitative PCR, and the results were in agreement with the RNA-Seq analysis. Our results may assist the development of new methods to decrease the albino seedling rates in wheat anther cultures.
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影响因子：
14.8
作者：
Chou, Kuo-Chen;Shen, Hong-Bin
通讯作者：
Shen, Hong-Bin
影响因子：
--
作者：
Liu JX;Chiou CY;Shen CH;Chen PJ;Liu YC;Jian CD;Shen XL;Shen FQ;Yeh KW
通讯作者：
Yeh KW
影响因子：
6.2
作者：
Gaomiao Zhan;Rong-Jun Li;Zhi-yong Hu;Jing Liu;Linbin Deng;Shi-you Lu;Wei Hua
通讯作者：
Gaomiao Zhan;Rong-Jun Li;Zhi-yong Hu;Jing Liu;Linbin Deng;Shi-you Lu;Wei Hua
影响因子：
2
作者：
ANDERSEN, SB;DUE, IK;OLESEN, A
通讯作者：
OLESEN, A
影响因子：
2
作者：
G. Ziegler;K. Dressler;D. Hess
通讯作者：
G. Ziegler;K. Dressler;D. Hess