Identification and characterization of a pore-forming protein of human peripheral blood natural killer cells.
Identification and characterization of a pore-forming protein of human peripheral blood natural killer cells.
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DOI：
10.1084/jem.164.6.2061
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发表时间：
1986-12-01
期刊：
影响因子：
--
通讯作者：
Young JD
中科院分区：
文献类型：
--
作者：
Liu CC;Perussia B;Cohn ZA;Young JD
我们在这里表明，人外周血NK细胞含有一种孔隙形成蛋白（PFP），其Mr值为70,000-72,000，可组装结构性病变（平均内径为150-170 a）并形成功能性通道。采用亲和层析法从人NK细胞中分离出PFP，采用特异性抗c9抗血清作为免疫吸附剂。利用一组针对不同NK细胞和T细胞表面抗原的单克隆抗体，通过阳性或阴性选择间接结簇法从PBL中分离NK细胞。在新分离的NK细胞和从培养的PBL中分离的NK细胞中发现了PFP，两者都受到白细胞介素2的刺激，但在NK细胞耗尽的淋巴细胞中没有。在平面双分子层中，NK细胞衍生的PFP形成的通道具有很高的耐电压性，大多数通道一旦插入双分子层就会保持开放状态。在0.1 M NaCl条件下，这些通道的单位电导范围为0.3-1 nS。该通道对单价和二价离子的选择性较差。受钙离子载体A23187刺激的人NK细胞也释放PFP，这表明该蛋白与小鼠CTL细胞系产生的蛋白一样，可能在细胞介导的杀伤过程中类似地分泌。它在原代人NK细胞培养中的鉴定表明，该蛋白可能在NK细胞介导的杀伤中发挥积极作用。
We show here that human peripheral blood NK cells contain a pore- forming protein (PFP) with an Mr of 70,000-72,000 that assembles structural lesions (with an average internal diameter of 150-170 A) and forms functional channels. The PFP was isolated by affinity chromatography from human NK cells, using a specific anti-C9 antiserum as the immunoadsorbent. The NK cells were isolated from PBL by positive or negative selection by indirect rosetting using a panel of monoclonal antibodies directed against different NK and T cell surface antigens. PFP was identified in NK cells freshly isolated and isolated from cultured PBL, both stimulated with interleukin 2, but not in NK cell- depleted lymphocytes. In planar bilayers, the channels formed by the NK cell-derived PFP are highly voltage resistant, with most channels persisting in the open state once they have inserted into the bilayer. The unit conductances of these channels range 0.3-1 nS in 0.1 M NaCl. The channels show poor selectivity for monovalent and divalent ions. The PFP is also released from human NK cells stimulated with the calcium ionophore A23187, suggesting that this protein, like the one produced by murine CTL lines, may be similarly secreted during cell- mediated killing. Its identification in primary human NK cell cultures indicates that this protein may play an active role in NK cell-mediated killing.
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影响因子：
56.9
作者：
KUNG, PC;GOLDSTEIN, G;SCHLOSSMAN, SF
通讯作者：
SCHLOSSMAN, SF
影响因子：
20.3
作者：
LOZZIO, CB;LOZZIO, BB
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LOZZIO, BB
影响因子：
5.4
作者：
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通讯作者：
HERZENBERG, LA
影响因子：
11.4
作者：
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通讯作者：
TSCHOPP, J
影响因子：
15.3
作者：
DENNERT, G;PODACK, ER
通讯作者：
PODACK, ER