Transcriptomic characterization and curation of candidate neuropeptides regulating reproduction in the eyestalk ganglia of the Australian crayfish, Cherax quadricarinatus.
Transcriptomic characterization and curation of candidate neuropeptides regulating reproduction in the eyestalk ganglia of the Australian crayfish, Cherax quadricarinatus.
复制标题
DOI：
10.1038/srep38658
复制
发表时间：
2016-12-07
影响因子：
4.6
通讯作者：
Ventura T
中科院分区：
文献类型：
--
作者：
Nguyen TV;Cummins SF;Elizur A;Ventura T
澳大利亚红螯螯虾（Cherax quadricarinatus）最近作为澳大利亚和全世界可持续水产养殖生产的新兴候选者受到关注。更重要的是，C。quadricarinatus是具有重要商业价值的十足类甲壳类动物的良好模式生物，因为它分布在世界各地，易于在实验室中维持，并且其生殖周期已得到很好的记录。为了更好地了解十足类甲壳动物生殖和发育的关键调控机制，目前已有的模式生物如C。quadricarinatus必须扩大。然而，目前还没有研究确定C。四脊肌神经肽组在这里，我们报告了一个全面的研究神经肽基因表达的眼柄在澳大利亚小龙虾C。四脊肌我们根据其他物种神经肽的关键特征，鉴定了53种推定的神经肽编码转录本。其中，14个神经肽参与生殖调节被选为评估其组织分布使用RT-PCR。进一步的见解进行了讨论，在其他物种和潜在的后续研究的神经肽的现有知识。总的来说，所得的数据奠定了基础，为未来的基因为基础的神经内分泌研究在C。四脊肌
The Australian redclaw crayfish (Cherax quadricarinatus) has recently received attention as an emerging candidate for sustainable aquaculture production in Australia and worldwide. More importantly, C. quadricarinatus serves as a good model organism for the commercially important group of decapod crustaceans as it is distributed worldwide, easy to maintain in the laboratory and its reproductive cycle has been well documented. In order to better understand the key reproduction and development regulating mechanisms in decapod crustaceans, the molecular toolkit available for model organisms such as C. quadricarinatus must be expanded. However, there has been no study undertaken to establish the C. quadricarinatus neuropeptidome. Here we report a comprehensive study of the neuropeptide genes expressed in the eyestalk in the Australian crayfish C. quadricarinatus. We characterised 53 putative neuropeptide-encoding transcripts based on key features of neuropeptides as characterised in other species. Of those, 14 neuropeptides implicated in reproduction regulation were chosen for assessment of their tissue distribution using RT-PCR. Further insights are discussed in relation to current knowledge of neuropeptides in other species and potential follow up studies. Overall, the resulting data lays the foundation for future gene-based neuroendocrinology studies in C. quadricarinatus.
登录
查看更多内容
影响因子：
0.8
作者：
Abdu, U;Yehezkel, G;Sagi, A
通讯作者：
Sagi, A
影响因子：
5.2
作者：
Choi MY;Vander Meer RK
通讯作者：
Vander Meer RK
影响因子：
3.7
作者：
Christie AE;Chi M;Lameyer TJ;Pascual MG;Shea DN;Stanhope ME;Schulz DJ;Dickinson PS
通讯作者：
Dickinson PS
DOI：
10.1016/0020-1790(87)90106-5
发表时间：
1987-01-01
期刊：
INSECT BIOCHEMISTRY
影响因子：
--
作者：
GIRARDIE, J;BOUREME, D;GIRARDIE, A
通讯作者：
GIRARDIE, A
青蟹大脑神经节中的神经肽，卵黄发生过程中的转录组分析和表达谱
影响因子：
4.6
作者：
Bao C;Yang Y;Huang H;Ye H
通讯作者：
Ye H