A multilayered scaffold of a chitosan and gelatin hydrogel supported by a PCL core for cardiac tissue engineering.
A multilayered scaffold of a chitosan and gelatin hydrogel supported by a PCL core for cardiac tissue engineering.
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DOI：
10.1016/j.actbio.2012.10.032
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发表时间：
2013-03
影响因子：
9.7
通讯作者：
Jacot, Jeffrey G.
中科院分区：
文献类型：
--
作者：
Pok, Seokwon;Myers, Jackson D.;Madihally, Sundararajan V.;Jacot, Jeffrey G.
将自组装的聚己内酯(PCL)夹在明胶-壳聚糖水凝胶中制成三维支架，作为一种可生物降解的补片，具有手术修复先天性心脏病的潜力。PCL核心提供了手术操作、可缝合性和高初始拉伸强度，而明胶-壳聚糖支架允许细胞附着，具有有利于心肌细胞迁移和功能的孔径大小和机械性能。由10、46和80 kDa(Mn)PCL共混物制成的PCL芯的极限拉应力可控制在2-4 Mpa范围内，平均相对分子质量较低的PCL共混物与较低的拉应力相关。低相对分子质量PCL的共混物也具有更快的降解速度(在30天内在生理盐水中的失重率可控制在0%至7%)，并具有更大的气孔。支持明胶-壳聚糖乳胶的PCL支架在拉伸应力、应变和拉伸模量方面没有明显的变化。然而，复合组织的压缩模数与天然组织相似(50%明胶和50%壳聚糖的∼为15kPa)。电子显微镜显示，明胶-壳聚糖凝胶具有三维多孔结构，平均孔径为∼80μm，显示了新生大鼠心肌细胞的迁移，并保持了7d以上的存活能力，并导致了自发跳动的支架。这种多层支架具有足够的抗张强度和手术处理能力，可用作心脏补片，同时允许心肌细胞在仿生环境中迁移或预加载，从而最终降解补片并整合到天然组织中。
A three-dimensional scaffold composed of self-assembled polycaprolactone (PCL) sandwiched in a gelatin–chitosan hydrogel was developed for use as a biodegradable patch with a potential for surgical reconstruction of congenital heart defects. The PCL core provides surgical handling, suturability and high initial tensile strength, while the gelatin–chitosan scaffold allows for cell attachment, with pore size and mechanical properties conducive to cardiomyocyte migration and function. The ultimate tensile stress of the PCL core, made from blends of 10, 46 and 80kDa (Mn) PCL, was controllable in the range of 2–4MPa, with lower average molecular weight PCL blends correlating with lower tensile stress. Blends with lower molecular weight PCL also had faster degradation (controllable from 0% to 7% weight loss in saline over 30days) and larger pores. PCL scaffolds supporting a gelatin–chitosan emulsion gel showed no significant alteration in tensile stress, strain or tensile modulus. However, the compressive modulus of the composite tissue was similar to that of native tissue (∼15kPa for 50% gelatin and 50% chitosan). Electron microscopy revealed that the gelatin–chitosan gel had a three-dimensional porous structure, with a mean pore diameter of ∼80μm, showed migration of neonatal rat ventricular myocytes (NRVM), maintained NRVM viability for over 7days, and resulted in spontaneously beating scaffolds. This multi-layered scaffold has sufficient tensile strength and surgical handling for use as a cardiac patch, while allowing migration or pre-loading of cardiac cells in a biomimetic environment to allow for eventual degradation of the patch and incorporation into native tissue.
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影响因子：
5.8
作者：
Grosvenor, MP;Staniforth, JN
通讯作者：
Staniforth, JN
影响因子：
2.4
作者：
Jacot JG;Martin JC;Hunt DL
通讯作者：
Hunt DL
影响因子：
14
作者：
Cheng, MY;Deng, JU;Zhang, XF
通讯作者：
Zhang, XF
影响因子：
14
作者：
Chi, Nai-Hsin;Yang, Ming-Chia;Wang, Shoei-Shen
通讯作者：
Wang, Shoei-Shen
DOI：
10.1111/j.1751-7133.2010.00145.x
发表时间：
2010-05
期刊：
Congestive heart failure (Greenwich, Conn.)
影响因子：
--
作者：
Kurdi M;Chidiac R;Hoemann C;Zouein F;Zgheib C;Booz GW
通讯作者：
Booz GW