In vitro human leukocyte labeling with (64)Cu: an intraindividual comparison with (111)In-oxine and (18)F-FDG.
In vitro human leukocyte labeling with (64)Cu: an intraindividual comparison with (111)In-oxine and (18)F-FDG.
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DOI：
10.1016/j.nucmedbio.2009.03.001
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发表时间：
2009-07
影响因子：
3.1
通讯作者：
Palestro, Christopher J.
中科院分区：
文献类型：
--
作者：
Bhargava, Kuldeep K.;Gupta, Raj K.;Nichols, Kenneth J.;Palestro, Christopher J.
我们研究了用~（64）Cu体外标记人白细胞（WBC），比较了Cu-WBC与~（111）In-oxine（In）WBC和~（18）F-FDG（FDG）WBC的标记效率、活性和稳定性。用Cu、In和FDG标记10名志愿者的白细胞。收集40 mL静脉血，并根据标准方法进行白细胞分离。根据公布的方法标记In-WBC和FDG-WBC。对于Cu-WBC，环庚三烯酚酮最初用作单一螯合剂。由于细胞内Cu保留较差（3小时时为54 ± 4%，24小时时为24 ± 5%），因此添加氟化的膜渗透性二价螯合剂quin-MF/AM。将WBC在含有100 μ L 1 mM quin-MF/AM的2%二甲亚砜溶液和74 - 185 MBq Cu-环庚三烯酚酮的5 mL盐水中于37 ℃孵育45分钟。测定标记效率、1、3和24小时的体外细胞活力以及1、2、3、4和24小时的体外稳定性（FDG-WBC除外）。平均Cu-WBC（87 ± 4%）和In-WBC（86 ± 4%）标记效率相当，显著高于FDG-WBC（60 ± 19%，p <0.001）。在1小时时相似的细胞活力在3小时和24小时时对于64Cu-WBC显著更高。In-WBC的细胞内活性保留始终显著高于Cu-WBC和FDG-WBC。24小时时，In-WBC的细胞内滞留率为88 ± 4%，Cu-WBC的细胞内滞留率为79 ± 6%。Cu-WBC的标记效率和活性与In-WBC相当或上级，显著高于FDG-WBC。尽管从Cu-WBC洗脱的活性显著高于从In-WBC洗脱的活性，但Cu-WBC可能足以用于成像。这些数据表明，进一步研究在体外铜-64标记的白细胞PET成像的感染是必要的。
We investigated labeling human leukocytes (WBC's) in vitro with copper-64 (Cu) comparing labeling efficiency, viability and stability of Cu-WBC's with 111In-oxine (In) WBC's and 18F-FDG (FDG) WBC's. Leukocytes from ten volunteers were labeled with Cu, In, and FDG. Forty mL venous blood was collected and leukocyte separation was performed according to standard methods. In-WBC's and FDG-WBC's were labeled according to published methods. For Cu-WBC's, tropolone initially was used as a single chelating agent. Because of poor intracellular Cu retention (54±4% at 3 hours, 24±5% at 24 hours), the fluorinated, membrane-permeable divalent chelator quin-MF/AM was added. WBC's were incubated in 5 mL saline containing 100 μL of 1mM quin-MF/AM in 2% dimethyl sulfoxide and 74-185 MBq Cu-tropolone for 45 minutes at 37°C. Labeling efficiencies, in-vitro cellular viabilities at 1, 3 and 24 hours, and in-vitro stabilities at 1,2,3,4 and 24 hours (except FDG-WBC's), were determined. Mean Cu-WBC's (87±4%) and In-WBC's (86±4%) labeling efficiencies were comparable and were significantly higher than FDG-WBC's (60±19%, p<0.001). Cell viabilities, similar at 1 hour, were significantly higher for 64Cu-WBC's at three and twenty-four hours. Intracellular retention of activity always was significantly higher for In-WBC's than Cu-WBC's and FDG-WBC's. At 24 hours, intracellular retention was 88±4%, for In-WBC's and 79±6% for Cu-WBC's. Cu-WBC's labeling efficiency and viability were comparable or superior to In-WBC's and significantly higher than FDG-WBC's. Although significantly more activity eluted from Cu-WBC's than from In-WBC's, Cu-WBC probably is adequate for imaging. These data suggest that further investigation of in vitro copper-64 labeled leukocytes for PET imaging of infection is warranted.
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影响因子：
1.5
作者：
DANPURE, HJ;OSMAN, S;CARROLL, MJ
通讯作者：
CARROLL, MJ
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