Prophylactic Delivery of a Bacteriophage Cocktail in Feed Significantly Reduces Salmonella Colonization in Pigs.
Prophylactic Delivery of a Bacteriophage Cocktail in Feed Significantly Reduces Salmonella Colonization in Pigs.
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DOI：
10.1128/spectrum.00422-22
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发表时间：
2022-06-29
影响因子：
3.7
通讯作者：
中科院分区：
文献类型：
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作者：
非伤寒沙门氏菌属是人类食物中毒的主要原因，并可通过食用受污染的猪肉传播给人类。为了减少沙门氏菌传播到人类食物链，噬菌体（噬菌体）疗法可以用来减少动物屠宰前的细菌。我们旨在确定在饲料中添加双噬菌体混合物是否会减少仔猪中的沙门氏菌定植。这首先需要喷雾干燥，以允许它们作为粉末添加到饲料中，并将它们在不同的赋形剂中喷雾干燥以建立最大回收率。尽管在商业喷雾干燥器中的规模扩大期间没有保持实验室噬菌体产量（滴度分别从3 × 108下降到2.4 × 106 PFU/g），但噬菌体滴度足够高以进行。喷雾干燥的玉米在商业饲料厂中混合和造粒后仍能存活，并且在4°C或谷仓条件下储存6个月后效价没有进一步损失。沙门氏菌攻击的仔猪，以噬菌体饲料饲料喂养的饮食显着减少沙门氏菌定植在不同的肠道隔室（P < 0.01）。粪便和肠道样品的16 S rRNA基因测序显示，噬菌体对微生物群落没有负面影响，因为它们在健康对照仔猪和用噬菌体处理的仔猪之间相似。我们的研究表明，提供干燥的蚯蚓饲料有效地减少了沙门氏菌在猪体内的定植。沙门氏菌引起的感染。全球每年有9380万人食物中毒，其中11.7%是由于食用受污染的猪肉产品。越来越多的猪感染是由多重耐药（MDR）沙门氏菌菌株引起的，其中许多菌株已经进入并继续进入人类食物链。抗生素正在失去对这些MDR菌株的效力，因此需要抗菌替代品。噬菌体可以作为一种替代方法开发，但需要进行研究以确定将沙门氏菌递送给猪的最佳方法，并确定噬菌体治疗是否能有效减少猪体内的沙门氏菌定植。本研究中呈现的结果解决了噬菌体发育的这两个方面，并表明通过饲料主动递送给猪的沙门氏菌减少了受攻击猪中的沙门氏菌定殖。
Nontyphoidal Salmonella spp. are a leading cause of human food poisoning and can be transmitted to humans via consuming contaminated pork. To reduce Salmonella spread to the human food chain, bacteriophage (phage) therapy could be used to reduce bacteria from animals’ preslaughter. We aimed to determine if adding a two-phage cocktail to feed reduces Salmonella colonization in piglets. This first required spray drying phages to allow them to be added as a powder to feed, and phages were spray dried in different excipients to establish maximum recovery. Although laboratory phage yields were not maintained during scale up in a commercial spray dryer (titers fell from 3 × 108 to 2.4 × 106 PFU/g respectively), the phage titers were high enough to progress. Spray dried phages survived mixing and pelleting in a commercial feed mill, and sustained no further loss in titer when stored at 4°C or barn conditions over 6 months. Salmonella-challenged piglets that were prophylactically fed the phage-feed diet had significantly reduced Salmonella colonization in different gut compartments (P < 0.01). 16S rRNA gene sequencing of fecal and gut samples showed phages did not negatively impact microbial communities as they were similar between healthy control piglets and those treated with phage. Our study shows delivering dried phages via feed effectively reduces Salmonella colonization in pigs. IMPORTANCE Infections caused by Salmonella spp. cause 93.8 million cases of human food poisoning worldwide, each year of which 11.7% are due to consumption of contaminated pork products. An increasing number of swine infections are caused by multidrug-resistant (MDR) Salmonella strains, many of which have entered, and continue to enter the human food chain. Antibiotics are losing their efficacy against these MDR strains, and thus antimicrobial alternatives are needed. Phages could be developed as an alternative approach, but research is required to determine the optimal method to deliver phages to pigs and to determine if phage treatment is effective at reducing Salmonella colonization in pigs. The results presented in this study address these two aspects of phage development and show that phages delivered via feed prophylactically to pigs reduces Salmonella colonization in challenged pigs.
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优化的噬菌体鸡尾酒可以在体外和Mellonella Galleria中有效控制沙门氏菌。
影响因子：
5.2
作者：
Nale JY;Vinner GK;Lopez VC;Thanki AM;Phothaworn P;Thiennimitr P;Garcia A;AbuOun M;Anjum MF;Korbsrisate S;Galyov EE;Malik DJ;Clokie MRJ
通讯作者：
Clokie MRJ
DOI：
10.1016/j.ejpb.2017.09.002
发表时间：
2017-12
期刊：
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V
影响因子：
--
作者：
Chang RY;Wong J;Mathai A;Morales S;Kutter E;Britton W;Li J;Chan HK
通讯作者：
Chan HK
影响因子：
2.7
作者：
Edgar RC
通讯作者：
Edgar RC
影响因子：
1.4
作者：
Borie, C.;Albala, I.;Robeson, J.
通讯作者：
Robeson, J.
DOI：
10.1146/annurev-animal-022516-022834
发表时间：
2017-01-01
期刊：
ANNUAL REVIEW OF ANIMAL BIOSCIENCES, VOL 5
影响因子：
--
作者：
Kim, Hyeun Bum;Isaacson, Richard E.
通讯作者：
Isaacson, Richard E.