Cloning, molecular characterization and expression analysis of heat shock cognate 70 (Hsc70) cDNA from turbot (Scophthalmus maximus)
Cloning, molecular characterization and expression analysis of heat shock cognate 70 (Hsc70) cDNA from turbot (Scophthalmus maximus)
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大菱鲆热休克同源 70 (Hsc70) cDNA 的克隆、分子表征和表达分析
DOI：
10.1007/s10695-013-9792-8
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发表时间：
2013-03
影响因子：
2.9
通讯作者：
Chen SL
中科院分区：
文献类型：
--
作者：
Wang TT;Wang N;Liao XL;Meng L;Liu Y;Chen SL
热休克相关蛋白70（Heat shock cognate 70，Hsc 70）是热休克蛋白70家族的重要成员，是一种组成型表达的分子伴侣，参与蛋白质代谢。本论文对大菱鲆Hsc 70基因进行了克隆和表达分析。结果表明，大菱鲆Hsc 70的cDNA全长为2，292 bp，其中5′ UTR为113 bp，3′ UTR为223 bp，开放阅读框为1，956 bp，编码651个氨基酸。氨基酸序列比较显示大菱鲆Hsc 70中存在3个经典的HSP 70家族特征基序、1个特征九肽和1个重复的四肽。大菱鲆Hsc 70编码的氨基酸序列与其它24个已知序列的Hsp 70 s/Hsc 70 s的同源性为75.4- 96.8%。特别是，发现最强的同源性与同源成员的Hsc 70亚家族和最高的一致性被发现与日本比目鱼Hsc 70。半定量RT-PCR结果表明，在正常生理条件下，turbotHsc 70基因在所有受试组织中均能稳定表达，而在热休克和细菌感染后，其表达量也增加了约1.5倍至约3倍。此外，Hsc 70在大菱鲆胚胎细胞系（TEC）中的表达在整个胚胎发育过程中都有表达。同时，TEC也是热诱导的，而不是冷诱导的。这些结果提示Hsc 70基因可能参与了正常和应激条件下的胚胎发生和细胞保护事件。
As an essential member of the HSP70 family, heat shock cognate 70 (Hsc70) is a constitutively expressed molecular chaperone involved in protein metabolism. In this paper, turbot Hsc70 was cloned and the expression profile was also analyzed. The full-length cDNA of the turbot Hsc70 was 2,292 bp in length, including a 113-bp 5′ UTR, a 223-bp 3′ UTR and a 1,956-bp open reading frame coding a protein with 651 amino acid residues. Comparison of amino acid sequence revealed the existence of three classical HSP70 family signature motifs, a signature nonapeptide and one repeat of tetrapeptide in turbot Hsc70. The turbot Hsc70-deduced amino acids sequence exhibited 75.4–96.8 % homology with Hsp70s/Hsc70s of 24 other known sequences. In particular, the strongest homology was found with the cognate members of Hsc70 subfamily and the highest identity was found with Japanese flounder Hsc70. Semi-quantitative RT-PCR revealed that turbotHsc70transcripts were stably expressed in all tested tissues under normal physiological condition, while the expression levels also increased (~1.5-fold to ~threefold) after heat shock and bacterial infection. In addition,Hsc70transcripts were detected throughout embryonic development and in turbot embryonic cell line (TEC) in the absence of any stress. Meanwhile, it was also heat inducible, but not cold inducible in TEC. These results suggest thatHsc70gene may be involved in embryogenesis and cellular protection events under normal and stress condition.
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影响因子：
6.7
作者：
V. Wilhelm;C. Soza;Rodrigo Martínez;M. Rosemblatt;L. Burzio;P. Valenzuela
通讯作者：
V. Wilhelm;C. Soza;Rodrigo Martínez;M. Rosemblatt;L. Burzio;P. Valenzuela
影响因子：
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作者：
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作者：
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通讯作者：
C. Bakkenist;J. Koreth;Cory SM Williams;N. Hunt;J. McGee
DOI：
10.1002/9781119432401.ch9
发表时间：
2020-02
期刊：
Heat Stress Tolerance in Plants
影响因子：
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作者：
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通讯作者：
Annu Yadav;Jitender Singh;K. Ranjan;Pankaj Kumar;Shivani Khanna;Madhuri Gupta;Vinay Kumar;S. H. Wani;Anil Sirohi
DOI：
10.1016/s1095-6433(03)00033-3
发表时间：
2003-04-01
影响因子：
2.3
作者：
Airaksinen, S;Råbergh, CMI;Nikinmaa, M
通讯作者：
Nikinmaa, M