Skills to Perform Vessel Eversion in Mouse Cervical Cardiac Transplantation with Cuff Technique.
Skills to Perform Vessel Eversion in Mouse Cervical Cardiac Transplantation with Cuff Technique.
复制标题
袖套法小鼠颈部心脏移植中血管翻转的技巧
DOI：
10.21470/1678-9741-2020-0125
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发表时间：
2021-06-01
影响因子：
1.3
通讯作者：
Peng L
中科院分区：
文献类型：
--
作者：
Tan L;Xie X;Xu Y;Tian Q;Zhang Q;Lan G;Wang H;Zhao Y;Peng L
小鼠异位心脏移植模型已被广泛用于研究移植免疫。虽然袖带技术有助于手术，但手术仍然困难，血管外翻是最困难的步骤。袖带移动和外翻血管壁滑动是血管外翻的主要不利因素。预防这些因素的传统策略集中在袖套固定，而需要更多的步骤或手术器械。根据报告的方案和我们的经验，血管外翻技术进行了改进，并用于移植。将来自C57 BL/6（H-2b）或BALB/c（H-2d）小鼠的心脏移植物移植到C57 BL/6（H-2b）小鼠中。对近90例手术的手术时间进行总结分析，按手术先后顺序分为9组。采用改良的血管外翻技术成功地进行了小鼠颈部心脏移植。袖带移动是防止血管外翻的最重要不利因素，因此得到有效防止。近90例手术中，手术时间47.3±7.9 min，成功率98%。改良的袖套法心脏移植术简化了血管外翻的操作，具有袖套移动少、操作步骤少、手术器械少等特点。使用这些手术技巧，移植可以在短时间内完成。
The mouse heterotopic cardiac transplant model has been extensively used to explore transplant immunity. Although the cuff technique facilitates the operation, the procedure remains difficult, and vessel eversion is the most difficult step. Cuff movement and everted vessel wall slippage are the main adverse factors in vessel eversion. Traditional strategies to prevent these factors focus on cuff fixation, while more steps or surgical instruments would be required. According to the reported protocols and our experience, the vessel eversion skills were modified and used for transplantation. Cardiac grafts from C57BL/6(H-2b) or BALB/c(H-2d) mice were transplanted into C57BL/6(H-2b) mice. The operating times of recent 90 operations, which were divided into 9 groups according to their sequence, were summarized and analyzed. The mouse cervical cardiac transplantation was successfully performed by using the modified vessel eversion skills. The cuff movement, which is the most important adverse factor to prevent vessel eversion, was effectively prevented. In the recent 90 operations, the total operating time was 47.3±7.9 min and the success rate was 98%. The modified surgical skills simplify the vessel eversion in mouse cervical cardiac transplantation with cuff technique, characterized by less cuff movement, fewer steps, and surgical instruments. Using these surgical skills, the transplant can be performed in a short time.
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影响因子：
1.2
作者：
Ratschiller, Thomas;Deutsch, Marcus-Andre;Krane, Markus
通讯作者：
Krane, Markus
影响因子：
6.2
作者：
Tomita, Y;Zhang, QW;Yasui, H
通讯作者：
Yasui, H
影响因子：
8.9
作者：
Tomita, Y;Zhang, QW;Yasui, H
通讯作者：
Yasui, H
影响因子：
6.2
作者：
CORRY, RJ;WINN, HJ;RUSSELL, PS
通讯作者：
RUSSELL, PS
影响因子：
2.1
作者：
Wang, QX;Liu, YS;Li, XK
通讯作者：
Li, XK