Protection of crucian carp (Carassius auratus Gibelio) against septicaemia caused by Aeromonas hydrophila using specific egg yolk immunoglobulins
Protection of crucian carp (Carassius auratus Gibelio) against septicaemia caused by Aeromonas hydrophila using specific egg yolk immunoglobulins
复制标题
使用特定的蛋黄免疫球蛋白保护鲫鱼（Carassius auratus Gibelio）免受嗜水气单胞菌引起的败血症
DOI：
10.1111/j.1365-2109.2012.03099.x
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发表时间：
2013-05
影响因子：
2
通讯作者：
Yongping Xu
中科院分区：
文献类型：
--
作者：
Liji Jin;Xiaoyu Li;Dongliang Zou;Shuying Li;Wenqian Song;Yongping Xu
用灭活的嗜水气单胞菌免疫产蛋鸡，获得卵黄免疫球蛋白（IgY）。纯化的IgY对A.对A.特异性IgY为75 mg mL−1。在随后的挑战试验中，100条鲤鱼（200~250 g）被分配到10个水箱中的一个，每个水箱10条鲤鱼。一个鱼缸中的鱼未受到攻击，而其余90条鱼腹腔内注射100 μL A。浓度为108 cfu mL−1的大肠杆菌。在接下来的21天里，所有的鱼都被转移到一个干净的水箱中，每天20分钟。四个槽中的鱼（一个未挑战槽和三个挑战槽）未接受处理，而其余六个槽中的鱼浸入0.5 g L-1水性非特异性IgY（N = 3）或0.5 g L-1水性特异性IgY（N = 3）中。血红蛋白浓度，白色和红细胞数量以及特定的IgY处理的鱼的死亡率与对照组有显着差异。这些结果表明，被动免疫浸泡与病原体特异性IgY可能提供了一个有价值的治疗。鲤鱼的寄生虫感染。
Egg yolk immunoglobulins (IgY) were obtained from laying hens immunized with inactivated Aeromonas hydrophila. The purified IgY was shown to inhibit the growth of A. hydrophila in vitro and the optimum concentration for inhibition of A. hydrophila-specific IgY was 75 mg mL−1. In a subsequent challenge trial, 100 carp (200~250 g) were assigned to one of ten tanks with ten carp per tank. The fish in one tank were unchallenged whereas the remaining 90 fish were injected intraperitoneally with 100 μL of A. hydrophila at a concentration of 108 cfu mL−1. For the next 21 days, all fish were moved in their respective groups to a clean tank for 20 min day−1. The fish in four tanks (one unchallenged tank and three challenged tanks) received no treatment whereas the fish in the remaining six tanks were immersed in either 0.5 g L−1 aqueous nonspecific IgY (N = 3) or 0.5 g L−1 aqueous specific IgY (N = 3). Haemoglobin concentrations, white and red blood cell numbers as well as the mortality of specific IgY-treated fish were significantly different from those of the control. These results suggest that passive immunization by immersion with pathogen-specific IgY may provide a valuable treatment for A. hydrophila infection in carp.
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影响因子：
2
作者：
A. Scott;W. Rogers
通讯作者：
A. Scott;W. Rogers
影响因子：
3.3
作者：
MEYER, FP
通讯作者：
MEYER, FP
影响因子：
2
作者：
K. Munkittrick;J. Leatherland
通讯作者：
K. Munkittrick;J. Leatherland
影响因子：
3.4
作者：
Austin, B;Austin, DA;Powell, R
通讯作者：
Powell, R
影响因子：
2.5
作者：
GUTIERREZ, MA;MIYAZAKI, T;KIM, M
通讯作者：
KIM, M