The spray-drying process is sufficient to inactivate infectious porcine epidemic diarrhea virus in plasma.
The spray-drying process is sufficient to inactivate infectious porcine epidemic diarrhea virus in plasma.
复制标题
DOI：
10.1016/j.vetmic.2014.09.008
复制
发表时间：
2014-11-07
影响因子：
3.3
通讯作者：
Opriessnig T
中科院分区：
文献类型：
--
作者：
Gerber PF;Xiao CT;Chen Q;Zhang J;Halbur PG;Opriessnig T
猪流行性腹泻病毒（PEDV）对养猪生产具有重要的经济意义。喷雾干燥的猪血浆与 PEDV 传播有关。在猪生物测定中测试了 PEDV 阳性猪血浆和 PEDV 加标血浆。喷雾干燥过程有效灭活了PEDV。通过喷雾干燥的猪血浆传播 PEDV 的风险很小。猪流行性腹泻病毒（PEDV）被认为是一种新出现的病原体，在许多养猪场造成巨大的经济损失。最近有人提出，通过将喷雾干燥猪血浆 (SDPP) 添加到保育猪日粮中，PEDV 可能会传播给幼猪群，这导致北美和欧洲的一些地区禁止使用 SDPP。为了确定喷雾干燥对 PEDV 感染性的影响，对 3 周龄的猪进行胃内接种：(1) 掺有 PEDV 的原始猪血浆 (RAW-PEDV-CONTROL)，(2) 掺有 PEDV 的猪血浆，然后喷雾干燥 (SD-PEDV-CONTROL)，(3) 来自 PEDV 感染猪的原始血浆 (RAW-SICK)，(4) 来自 PEDV 感染猪的喷雾干燥血浆(SD-SICK)，或 (5) PEDV 阴性猪的喷雾干燥血浆 (SD-NEG-CONTROL)。对于喷雾干燥过程，使用了具有类似工业入口和出口温度设置的台式喷雾干燥器。在 RAW-PEDV-CONTROL 组中，感染后第 3 天（dpi）时粪便中存在 PEDV RNA，猪在 dpi 14 时发生血清转化。相比之下，在其他组（包括 SD-PEDV-CONTROL 组）的任何猪中均未检测到粪便中的 PEDV RNA，并且在 dpi 28 时项目终止时没有一头猪发生血清转化。这项工作提供了直接证据，表明实验本研究中使用的喷雾干燥工艺可有效灭活血浆中的传染性 PEDV。此外，从疾病高峰期感染 PEDV 的猪身上收集的血浆不含有传染性 PEDV。这些发现表明，PEDV 通过商业生产的 SDPP 传播的风险很小。
Porcine epidemic diarrhea virus (PEDV) is economically important to pig production. Spray-dried pig plasma has been implicated in PEDV transmission. PEDV-positive pig plasma and PEDV-spiked plasma were tested in a swine bioassay. The spray-drying process effectively inactivated PEDV. The risk of transmitting PEDV through spray-dried pig plasma is minimal. Porcine epidemic diarrhea virus (PEDV) is considered an emergent pathogen associated with high economic losses in many pig rearing areas. Recently it has been suggested that PEDV could be transmitted to naïve pig populations through inclusion of spray-dried porcine plasma (SDPP) into the nursery diet which led to a ban of SDPP in several areas in North America and Europe. To determine the effect of spray-drying on PEDV infectivity, 3-week-old pigs were intragastrically inoculated with (1) raw porcine plasma spiked with PEDV (RAW-PEDV-CONTROL), (2) porcine plasma spiked with PEDV and then spray dried (SD-PEDV-CONTROL), (3) raw plasma from PEDV infected pigs (RAW-SICK), (4) spray-dried plasma from PEDV infected pigs (SD-SICK), or (5) spray-dried plasma from PEDV negative pigs (SD-NEG-CONTROL). For the spray-drying process, a tabletop spray-dryer with industry-like settings for inlet and outlet temperatures was used. In the RAW-PEDV-CONTROL group, PEDV RNA was present in feces at day post infection (dpi) 3 and the pigs seroconverted by dpi 14. In contrast, PEDV RNA in feces was not detected in any of the pigs in the other groups including the SD-PEDV-CONTROL group and none of the pigs had seroconverted by termination of the project at dpi 28. This work provides direct evidence that the experimental spray-drying process used in this study was effective in inactivating infectious PEDV in the plasma. Additionally, plasma collected from PEDV infected pigs at peak disease did not contain infectious PEDV. These findings suggest that the risk for PEDV transmission through commercially produced SDPP is minimal.
登录
查看更多内容
影响因子：
3.7
作者：
Opriessnig T;Xiao CT;Gerber PF;Zhang J;Halbur PG
通讯作者：
Halbur PG
影响因子：
2.7
作者：
Park SJ;Kim HK;Song DS;Moon HJ;Park BK
通讯作者：
Park BK
影响因子：
6.4
作者：
Huang YW;Dickerman AW;Piñeyro P;Li L;Fang L;Kiehne R;Opriessnig T;Meng XJ
通讯作者：
Meng XJ
影响因子：
4.3
作者：
Pasick J;Berhane Y;Ojkic D;Maxie G;Embury-Hyatt C;Swekla K;Handel K;Fairles J;Alexandersen S
通讯作者：
Alexandersen S
影响因子：
1.5
作者：
Kim, O;Chae, C;Kweon, CH
通讯作者：
Kweon, CH