Altered Gene Expression of the Parasitoid Pteromalus puparum after Entomopathogenic Fungus Beauveria bassiana Infection.
Altered Gene Expression of the Parasitoid Pteromalus puparum after Entomopathogenic Fungus Beauveria bassiana Infection.
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DOI：
10.3390/ijms242317030
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发表时间：
2023-12-01
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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作者：
寄生蜂和昆虫病原真菌对于管理害虫种群正变得越来越重要。因此，有必要仔细考虑昆虫病原真菌对寄生蜂的潜在影响，因为它们具有广泛的致病性，而且在田间应用时，这些生物防治工具之间可能存在重叠。然而，尽管昆虫病原真菌对寄生蜂的致病性很重要，但很少有人研究它们对寄生蜂的致病性。在我们的研究中，我们的目的是通过调查著名的昆虫病原真菌球孢白僵菌和幼虫内寄生蜂Pteromalus puparum之间的相互作用来解决这一知识差距。我们的结果表明，在实验室条件下，球孢白僵菌的存在显著影响了幼虫的存活率。通过表面喷洒和口服测定，球孢白僵菌对蚕蛾的致病力具有剂量和时间依赖性。RNA-Seq分析表明，免疫系统在防御球孢白僵菌中起着主要和关键的作用。值得注意的是，在昆虫免疫系统的关键组成部分Toll和IMD途径中，几个上调的差异表达基因(DIG)和抗菌肽在感染的早期和晚期都被迅速诱导。相反，参与酚氧化酶原激活和抗氧化机制的大多数基因都下调了。此外，我们确定了与角质层形成、嗅觉机制和解毒过程相关的下调DEG。综上所述，我们的研究为了解蚕蛾和球孢杆菌之间的相互作用提供了有价值的见解，为了解真菌感染过程中基因表达的变化提供了线索。这些发现对制定更有效和可持续的农业病虫害管理战略具有重要意义。
Both parasitoids and entomopathogenic fungi are becoming increasingly crucial for managing pest populations. Therefore, it is essential to carefully consider the potential impact of entomopathogenic fungi on parasitoids due to their widespread pathogenicity and the possible overlap between these biological control tools during field applications. However, despite their importance, little research has been conducted on the pathogenicity of entomopathogenic fungi on parasitoids. In our study, we aimed to address this knowledge gap by investigating the interaction between the well-known entomopathogenic fungus Beauveria bassiana, and the pupal endoparasitoid Pteromalus puparum. Our results demonstrated that the presence of B. bassiana significantly affected the survival rates of P. puparum under laboratory conditions. The pathogenicity of B. bassiana on P. puparum was dose- and time-dependent, as determined via through surface spraying or oral ingestion. RNA-Seq analysis revealed that the immune system plays a primary and crucial role in defending against B. bassiana. Notably, several upregulated differentially expressed genes (DEGs) involved in the Toll and IMD pathways, which are key components of the insect immune system, and antimicrobial peptides were rapidly induced during both the early and late stages of infection. In contrast, a majority of genes involved in the activation of prophenoloxidase and antioxidant mechanisms were downregulated. Additionally, we identified downregulated DEGs related to cuticle formation, olfactory mechanisms, and detoxification processes. In summary, our study provides valuable insights into the interactions between P. puparum and B. bassiana, shedding light on the changes in gene expression during fungal infection. These findings have significant implications for the development of more effective and sustainable strategies for pest management in agriculture.
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DOI：
10.3390/pathogens12070957
发表时间：
2023-07-20
期刊：
Pathogens (Basel, Switzerland)
影响因子：
--
作者：
通讯作者：
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影响因子：
4.2
作者：
Hansen, Lise Stengard;Steenberg, Tove
通讯作者：
Steenberg, Tove
影响因子：
2.8
作者：
Feng, MG;Pu, XY;Wang, YG
通讯作者：
Wang, YG
影响因子：
2.2
作者：
Cai, J;Ye, GY;Hu, C
通讯作者：
Hu, C
影响因子：
4.8
作者：
Ibarra-Cortes, Karla H.;Gonzalez-Hernandez, Hctor;Robles-Bermudez, Agustin
通讯作者：
Robles-Bermudez, Agustin