Bioactive stent surface coating that promotes endothelialization while preventing platelet adhesion.
Bioactive stent surface coating that promotes endothelialization while preventing platelet adhesion.
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DOI：
10.1021/bm101212k
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发表时间：
2011-03-14
影响因子：
6.2
通讯作者：
Grinstaff, Mark W.
中科院分区：
文献类型：
--
作者：
Meyers, Steven R.;Kenan, Daniel J.;Khoo, Xiaojuan;Grinstaff, Mark W.
设计、合成并评价了双功能肽涂层作为潜在的促愈合支架涂层。双功能肽由一个短的28-mer序列组成，在N-末端具有对聚苯乙烯结合具有亲和力的基序，在C-末端具有显示选择性结合人内皮细胞但不结合血小板的基序。结果表明，选择性涂层，聚苯乙烯结合肽终止于RRETAWA（FFSFFFPASAWGSSGSSGK（生物素）CRRETAWAC），结合内皮细胞定量以及常见的RGD基序，但不像RGD，它没有显示任何偏好血小板粘附。后续工作检查了内皮细胞（其结合应被鼓励）和平滑肌细胞（其结合应在支架应用中被弃用）之间的细胞系选择性差异，确实确定了RRETAWA终止的肽涂层对内皮细胞的时间偏好。然而，在得出明确的结论之前，需要更详细地检查这种明显的选择性在体内的影响。积极的体外结果鼓励继续开发其他新型涂层，模拟生物结构和/或信号传导能力，以指导合成材料表面上的细胞过程。
A bifunctional peptide coating was designed, synthesized, and evaluated as a potential pro-healing stent coating. The bifunctional peptide consisted of a short 28-mer sequence that on the N-terminus, has a motif with affinity for polystyrene binding, and at the C-terminus, a motif that was shown to selectively bind human endothelial cells but not platelets. Results showed that the selective coating, a polystyrene binding peptide terminated in RRETAWA (FFSFFFPASAWGSSGSSGK(biotin)CRRETAWAC), bound endothelial cells quantitatively as well as the common RGD motif, but unlike RGD, it did not show any preference for platelet adherence. Follow-up work examining the difference in cell line selectivity between endothelial cells, whose binding should be encouraged, and smooth muscle cells, whose binding should be deprecated in a stenting application, did identify a temporal preference of the RRETAWA-terminated peptide coating for endothelial cells. However, the in vivo implications of this apparent selectivity need to be examined in more detail before definitive conclusions can be drawn. The positive in vitro results encourage the continued development of other novel coatings that mimic biological structures and/or signaling capabilities to direct cellular processes on the surface of synthetic materials.
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影响因子：
--
作者：
Kenan, Daniel J.;Walsh, Elisabeth B.;Grinstaff, Mark W.
通讯作者：
Grinstaff, Mark W.
影响因子：
29.4
作者：
Meyers, Steven R.;Hamilton, Paul T.;Grinstaff, Mark W.
通讯作者：
Grinstaff, Mark W.
影响因子：
2.3
作者：
Miglionico, Marco;Patti, Giuseppe;Di Sciascio, Germano
通讯作者：
Di Sciascio, Germano
影响因子：
4.3
作者：
Germanier, Y;Tosatti, S;Buser, D
通讯作者：
Buser, D
影响因子：
14
作者：
Meyers, Steven R.;Khoo, Xiaojuan;Kenan, Daniel J.
通讯作者：
Kenan, Daniel J.